PHA-L lectin and carbohydrate relationship: conjugation with CdSe/CdS nanoparticles, radiolabeling and in vitro affinities on MCF-7 cells

This research aims to investigate the interaction between phytohemagglutinin-L (PHA-L) and sialic acid, which is abundant on the breast cancer cell (MCF-7) surface and displays monosaccharide characteristics, by experimental and computational methods. Experimentally, CdSe/CdS nanoparticles (QDs) wer...

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Bibliographic Details
Published in:Journal of radioanalytical and nuclear chemistry 2014, Vol.299 (1), p.807-813
Main Authors: Kara, Altan, Ünak, Perihan, Selçuki, Cenk, Akça, Özlet, Medine, E. İlker, Sakarya, Serhan
Format: Article
Language:English
Subjects:
Affinity
Cadmium selenides
Carbohydrates
Chemistry
Chemistry and Materials Science
Computation
Crystal structure
Diagnostic Radiology
Fructose
Hadrons
Heavy Ions
In vitro testing
Inorganic Chemistry
Intermetallics
Lectins
Mathematical analysis
Nanoparticles
Nuclear Chemistry
Nuclear Physics
Organic acids
Physical Chemistry
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Summary:This research aims to investigate the interaction between phytohemagglutinin-L (PHA-L) and sialic acid, which is abundant on the breast cancer cell (MCF-7) surface and displays monosaccharide characteristics, by experimental and computational methods. Experimentally, CdSe/CdS nanoparticles (QDs) were synthesized; PHA-L was conjugated with QDs and labeled with 125 I. Radiolabeling yield was found to be 97 ± 1.2 %. Afterwards, in vitro bioaffinities of radiolabeled PHA-L conjugated QDs have been investigated on MCF-7 cells and it has been observed that the cell incorporation increased with time. The results indicated that 125 I labeled QD-PHA-L conjugates represent significant affinity on MCF-7 cells. In the second step of the study, the crystal structure of carbohydrate interaction surface of PHA-L was extracted from the crystal structure of PHA-L. The interactions between this surface and sialic acid were calculated by computational tools. These calculations revealed specific interactions between PHA-L and sialic acid. Semi-empirical methods, PM3 and AM1, were used in these calculations. Significant outcomes have been obtained from the experimental and computational studies and these results demonstrated that PHA-L may be an effective agent for imagining MCF-7 cells.
ISSN:0236-5731
1588-2780
DOI:10.1007/s10967-013-2783-5