The expression of Akt kinase in the heart ventricles under hypoxic preconditioning and myocardial remodeling

Activation of Akt-dependent mechanisms may play a significant role in the cellular response under hypoxic preconditioning and myocardial remodeling. The impact of hypoxic preconditioning, and remodeling on the expression of Akt kinase in the heart ventricles was investigated. Wistar male rats, the r...

Full description

Saved in:
Bibliographic Details
Published in:Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Ukraine : 1994), 2013, Vol.59 (6), p.124-131
Main Authors: Portnichenko, A G, Lapikova-Briginskaia, T Iu, Vasilenko, M I, Portnichenko, G V, Maslov, L N, Moĭbenko, A A
Format: Article
Language:Russian
Subjects:
Adaptation, Physiological
Altitude
Animals
Blotting, Western
Heart Ventricles - enzymology
Heart Ventricles - pathology
Hypoxia - enzymology
Hypoxia - pathology
Hypoxia - physiopathology
Male
Proto-Oncogene Proteins c-akt - biosynthesis
Rats
Rats, Wistar
Time Factors
Ventricular Remodeling - physiology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Activation of Akt-dependent mechanisms may play a significant role in the cellular response under hypoxic preconditioning and myocardial remodeling. The impact of hypoxic preconditioning, and remodeling on the expression of Akt kinase in the heart ventricles was investigated. Wistar male rats, the residents of plains or middle altitude (2100 m above sea level), were exposed to hypoxic preconditioning by "lifting" in the barochamber at the "height" of 5,600 m in 3 h. In the right and left ventricles of the heart, Akt protein expression was determined by Western blotting. It was shown, that hypoxic preconditioning causes the induction of Akt kinase in the ventricles during the period of delayed cardioprotection (1-3 days after preconditioning). Myocardial remodeling induced by chronic hypoxia in middle altitude was associated with elevated Akt expression in the myocardium, more pronounced in the left ventricle. Progression of hypoxic myocardial remodeling found in part of the animals was accompanied by a reduction of the cell hypoxic reactivity, including Akt induction in response to preconditioning. Thus, Akt kinase is involved in the mechanisms of hypoxia induced late preconditioning and myocardial remodeling in chronic hypoxia. Inhibitory regulatory mechanism was found to limit the induction of Akt in myocardium after remodeling.
ISSN:2522-9028