Expression of Natural Cytotoxicity Receptors on Peritoneal Fluid Natural Killer Cell and Cytokine Production by Peritoneal Fluid Natural Killer Cell in Women with Endometriosis

Problem To investigate the relationship between the expression of natural cytotoxicity receptors (NCRs) on peritoneal fluid (PF) natural killer (NK) (pfNK) cells and cytokine production by pfNK cells in women with endometriosis. Method of study Peritoneal fluid was collected from women with endometr...

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Bibliographic Details
Published in:American journal of reproductive immunology (1989) 2014-04, Vol.71 (4), p.359-367
Main Authors: Funamizu, Ayano, Fukui, Atsushi, Kamoi, Mai, Fuchinoue, Kohei, Yokota, Megumi, Fukuhara, Rie, Mizunuma, Hideki
Format: Article
Language:English
Subjects:
Adult
Antigens, Surface - biosynthesis
Ascitic Fluid - immunology
Cell Proliferation
Cytokine
Cytokines - biosynthesis
endometriosis
Endometriosis - immunology
Endometriosis - pathology
Female
Humans
Interferon-gamma - biosynthesis
Killer Cells, Natural - immunology
Laparoscopy
Natural Cytotoxicity Triggering Receptor 1 - biosynthesis
natural killer cell
Neovascularization, Pathologic
NKp46
peritoneal fluid
Receptors, Natural Cytotoxicity Triggering - biosynthesis
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:Problem To investigate the relationship between the expression of natural cytotoxicity receptors (NCRs) on peritoneal fluid (PF) natural killer (NK) (pfNK) cells and cytokine production by pfNK cells in women with endometriosis. Method of study Peritoneal fluid was collected from women with endometriosis undergoing laparoscopic surgery (n = 21) and controls without endometriosis (n = 28). The expression of NK cell surface antigens such as CD16 and NCRs (NKp46, NKp44 and NKp30) on pfNK cells, and cytokines production by pfNK cells [tumor necrosis factor (TNF)‐α, IFN‐γ, IL‐4, IL‐10, GM‐CSF and transforming growth factor (TGF)‐β1] were measured using multicolor flow cytometry. Results The percentages of CD56+/NKp46+ cells and CD56dim/NKp46+ cells in severe endometriosis group were significantly lower than that in controls. TNF‐α and IFN‐γ production by pfNK cells in severe endometriosis group was significantly higher than those in controls. Conclusion The differential expression of NKp46, TNF‐α, and IFN‐γ on pfNK cells in women with severe endometriosis may allow the proliferation and angiogenesis of endometriotic cells.
ISSN:1046-7408
1600-0897
DOI:10.1111/aji.12206