Isolation and characterization of a novel bacterial strain Shewanella marinisediminis sp. nov. from deep-sea sediments

A new bacterial strain designated DH39T was isolated from marine sediment collected from the East Sea, Korea. Phylogenetic analysis using the 16S rRNA gene sequence revealed that strain DH39(T) clustered with the genus Shewanella and is closely related to Shewanella canadensis HAW-EB2(T), S. woodyi...

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Bibliographic Details
Published in:Journal of environmental biology 2013-05, Vol.34 (3), p.501-501
Main Authors: Kim, Young Sug, Park, Jin-Soo, Yang, Hyun Ok, Kwon, Hak Cheol
Format: Article
Language:English
Subjects:
Bacteria
Deep sea
Deoxyribonucleic acid
DNA
Environmental science
Genes, Bacterial
Geologic Sediments - microbiology
Marine Biology
Marine sediments
Microscopy, Electron, Scanning
Phylogeny
RNA, Ribosomal, 16S - genetics
Shewanella
Shewanella - classification
Shewanella - genetics
Shewanella - isolation & purification
Water Microbiology
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Summary:A new bacterial strain designated DH39T was isolated from marine sediment collected from the East Sea, Korea. Phylogenetic analysis using the 16S rRNA gene sequence revealed that strain DH39(T) clustered with the genus Shewanella and is closely related to Shewanella canadensis HAW-EB2(T), S. woodyi MS32(T), and S. sediminis HAW-EB3(T) with 98.1, 97.8, and 97.6% sequence similarities, respectively. The isolated bacterium was Gram-negative, rod-shaped, and aerobic. Its temperature range for growth was 4-30 degrees C. The predominant fatty acids were 16:1omega7, 17:1omega8, 13:0-i, 16:0, and 15:0-i. The DNA G+C content was 45.0 mol%. DNA-DNA hybridization analysis showed that DNA-DNA relatedness values in the 165 rRNA phylogenetic tree of strain DH39(T) and its nearest neighbors S. hanedai and S. sediminis were 52.9 and 58.7%. Phylogenetic evidence and phenotypic characteristics suggest strain DH39(T) constitutes a novel Shewanella species. Therefore, we propose Shewanella marinisediminis sp. nov., with DH39(T) (KCCM 42936(T) = NCCB 100311(T)) as the type strain.
ISSN:0254-8704
2394-0379