Loading…
Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway
Objective To evaluate the effect of Bear Bile Powder(熊胆粉, BBP) on the growth and apoptosis of HepG2 human hepatocellular carcinoma cells, and investigate the possible molecular mechanisms mediating its anti-cancer activity. Methods HepG2 cells were treated with 0.4–1.0 mg/mL of BBP for 24, 48 and 72...
Saved in:
| Published in: | Chinese journal of integrative medicine 2014-02, Vol.20 (2), p.123-129 |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c344t-9c1cc843b9371e146d8a732f270e5a7781c2f6029cfc48ff40d5ffa6798b30253 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c344t-9c1cc843b9371e146d8a732f270e5a7781c2f6029cfc48ff40d5ffa6798b30253 |
| container_end_page | 129 |
| container_issue | 2 |
| container_start_page | 123 |
| container_title | Chinese journal of integrative medicine |
| container_volume | 20 |
| creator | Zhao, Jin-yan Chen, Zhi-hong Lin, Wei Zhong, Xiao-yong Chen, Xu-zheng Peng, Jun Hong, Zhen-feng |
| description | Objective
To evaluate the effect of Bear Bile Powder(熊胆粉, BBP) on the growth and apoptosis of HepG2 human hepatocellular carcinoma cells, and investigate the possible molecular mechanisms mediating its anti-cancer activity.
Methods
HepG2 cells were treated with 0.4–1.0 mg/mL of BBP for 24, 48 and 72 h. The viability of HePG2 cells was determined by MTT assay. Cellular morphology was observed via phase-contrast microscopy. Fluorescence-activated cell sorting analysis with Annexin-V/propidium idodide and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazol-carbocyanine iodide (JC-1) staining was performed to determine cell apoptosis and the loss of mitochondrial membrane potential, respectively. Activation of caspase-9 and -3 was evaluated by a colorimetric assay.
Results
The treatment with 0.4–1 mg/mL of BBP for 24, 48, or 72 h respectively reduced cell viability significantly by 7%–60%, 20%–90% or 25%–98%, compared with the untreated control cells (
P |
| doi_str_mv | 10.1007/s11655-013-1581-9 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1507189459</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1507189459</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-9c1cc843b9371e146d8a732f270e5a7781c2f6029cfc48ff40d5ffa6798b30253</originalsourceid><addsrcrecordid>eNp9kM1OFjEUhhuiAQQugI3pEhejPf2ZTpdI_EtI3Oi66ddp-Upm2rGdgcDWhEQvx5vCy7CTD1266kn7vG9OH4ROgbwGQuSbAtAK0RBgDYgOGrWHDkEp1hBO6LM6t5LWGcQBelHKNSFCtkTsowPKW1CUyUN0_9aZjDdhcHhKt73L-Ozx4efv7w-Pv368wiH2i3UFmylNcyqh4OTxdhlNxFs3mTlZNwzLUBusyTbENBq8XhV8EwweQwW2KfY5pNj0bnKxd3HGNbi9NXfH6Lk3Q3EnT-cR-vr-3ZeLj83l5w-fLs4vG8s4nxtlwdqOs41iEhzwtu-MZNRTSZwwUnZgqW8JVdZb3nnPSS-8N61U3YYRKtgROtv1Tjl9W1yZ9RjKuqWJLi1FgyASOsWFqijsUJtTKdl5PeUwmnyngehVud4p11W5XpXrNfPyqX7ZjK7_l_jruAJ0B5T6FK9c1tdpybF--T-tfwDBmpBb</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1507189459</pqid></control><display><type>article</type><title>Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway</title><source>Springer Nature</source><creator>Zhao, Jin-yan ; Chen, Zhi-hong ; Lin, Wei ; Zhong, Xiao-yong ; Chen, Xu-zheng ; Peng, Jun ; Hong, Zhen-feng</creator><creatorcontrib>Zhao, Jin-yan ; Chen, Zhi-hong ; Lin, Wei ; Zhong, Xiao-yong ; Chen, Xu-zheng ; Peng, Jun ; Hong, Zhen-feng</creatorcontrib><description>Objective
To evaluate the effect of Bear Bile Powder(熊胆粉, BBP) on the growth and apoptosis of HepG2 human hepatocellular carcinoma cells, and investigate the possible molecular mechanisms mediating its anti-cancer activity.
Methods
HepG2 cells were treated with 0.4–1.0 mg/mL of BBP for 24, 48 and 72 h. The viability of HePG2 cells was determined by MTT assay. Cellular morphology was observed via phase-contrast microscopy. Fluorescence-activated cell sorting analysis with Annexin-V/propidium idodide and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazol-carbocyanine iodide (JC-1) staining was performed to determine cell apoptosis and the loss of mitochondrial membrane potential, respectively. Activation of caspase-9 and -3 was evaluated by a colorimetric assay.
Results
The treatment with 0.4–1 mg/mL of BBP for 24, 48, or 72 h respectively reduced cell viability significantly by 7%–60%, 20%–90% or 25%–98%, compared with the untreated control cells (
P
<0.01). In addition, BBP treatment induced morphological changes in HepG2 cells. Furthermore, after treated with 0, 0.4, 0.6, 0.8 and 1.0 mg/mL of BBP, apoptosis cells (including early and late apoptotic cells) were 18.0%±1.3%, 34.9%±2.2%, 33.9%±2.8%, 37.4%±2.8% and 46.0%±2.5%, respectively (
P
<0.05); and the percentage of cells with reduced JC-1 red fluorescence were 6.6%±0.8%, 8.5%±0.8%, 13.5%±1.6%, 17.6%±2.3% and 46.7%±3.6%, respectively (
P
<0.01). Finally, BBP treatment significantly and dose-dependently induced activation of both caspase-9 and caspase-3 in HepG2 cells (
P
<0.05).
Conclusions
BBP could inhibit the growth of HepG2 hepatocellular cancer cells through mitochondrion-mediated apoptosis, which may, in part, explain its anti-cancer activity. BBP may be a potential novel therapeutic agent for the treatment of hepatocellular carcinoma.</description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><identifier>DOI: 10.1007/s11655-013-1581-9</identifier><identifier>PMID: 24619237</identifier><language>eng</language><publisher>Heidelberg: Chinese Association of Traditional and Western Medicine</publisher><subject>Animals ; Apoptosis - drug effects ; Bile ; Carcinoma, Hepatocellular - drug therapy ; Carcinoma, Hepatocellular - pathology ; Caspases - metabolism ; Cell Proliferation - drug effects ; Cell Shape - drug effects ; Cell Survival - drug effects ; Drugs, Chinese Herbal - pharmacology ; Drugs, Chinese Herbal - therapeutic use ; Hep G2 Cells ; Humans ; Liver Neoplasms - drug therapy ; Liver Neoplasms - pathology ; Medicine ; Medicine & Public Health ; Membrane Potential, Mitochondrial - drug effects ; Mitochondria - drug effects ; Mitochondria - metabolism ; Original Article ; Signal Transduction - drug effects ; Ursidae</subject><ispartof>Chinese journal of integrative medicine, 2014-02, Vol.20 (2), p.123-129</ispartof><rights>Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-9c1cc843b9371e146d8a732f270e5a7781c2f6029cfc48ff40d5ffa6798b30253</citedby><cites>FETCH-LOGICAL-c344t-9c1cc843b9371e146d8a732f270e5a7781c2f6029cfc48ff40d5ffa6798b30253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24619237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Jin-yan</creatorcontrib><creatorcontrib>Chen, Zhi-hong</creatorcontrib><creatorcontrib>Lin, Wei</creatorcontrib><creatorcontrib>Zhong, Xiao-yong</creatorcontrib><creatorcontrib>Chen, Xu-zheng</creatorcontrib><creatorcontrib>Peng, Jun</creatorcontrib><creatorcontrib>Hong, Zhen-feng</creatorcontrib><title>Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway</title><title>Chinese journal of integrative medicine</title><addtitle>Chin. J. Integr. Med</addtitle><addtitle>Chin J Integr Med</addtitle><description>Objective
To evaluate the effect of Bear Bile Powder(熊胆粉, BBP) on the growth and apoptosis of HepG2 human hepatocellular carcinoma cells, and investigate the possible molecular mechanisms mediating its anti-cancer activity.
Methods
HepG2 cells were treated with 0.4–1.0 mg/mL of BBP for 24, 48 and 72 h. The viability of HePG2 cells was determined by MTT assay. Cellular morphology was observed via phase-contrast microscopy. Fluorescence-activated cell sorting analysis with Annexin-V/propidium idodide and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazol-carbocyanine iodide (JC-1) staining was performed to determine cell apoptosis and the loss of mitochondrial membrane potential, respectively. Activation of caspase-9 and -3 was evaluated by a colorimetric assay.
Results
The treatment with 0.4–1 mg/mL of BBP for 24, 48, or 72 h respectively reduced cell viability significantly by 7%–60%, 20%–90% or 25%–98%, compared with the untreated control cells (
P
<0.01). In addition, BBP treatment induced morphological changes in HepG2 cells. Furthermore, after treated with 0, 0.4, 0.6, 0.8 and 1.0 mg/mL of BBP, apoptosis cells (including early and late apoptotic cells) were 18.0%±1.3%, 34.9%±2.2%, 33.9%±2.8%, 37.4%±2.8% and 46.0%±2.5%, respectively (
P
<0.05); and the percentage of cells with reduced JC-1 red fluorescence were 6.6%±0.8%, 8.5%±0.8%, 13.5%±1.6%, 17.6%±2.3% and 46.7%±3.6%, respectively (
P
<0.01). Finally, BBP treatment significantly and dose-dependently induced activation of both caspase-9 and caspase-3 in HepG2 cells (
P
<0.05).
Conclusions
BBP could inhibit the growth of HepG2 hepatocellular cancer cells through mitochondrion-mediated apoptosis, which may, in part, explain its anti-cancer activity. BBP may be a potential novel therapeutic agent for the treatment of hepatocellular carcinoma.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Bile</subject><subject>Carcinoma, Hepatocellular - drug therapy</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Caspases - metabolism</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Shape - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Drugs, Chinese Herbal - therapeutic use</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Liver Neoplasms - drug therapy</subject><subject>Liver Neoplasms - pathology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Original Article</subject><subject>Signal Transduction - drug effects</subject><subject>Ursidae</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kM1OFjEUhhuiAQQugI3pEhejPf2ZTpdI_EtI3Oi66ddp-Upm2rGdgcDWhEQvx5vCy7CTD1266kn7vG9OH4ROgbwGQuSbAtAK0RBgDYgOGrWHDkEp1hBO6LM6t5LWGcQBelHKNSFCtkTsowPKW1CUyUN0_9aZjDdhcHhKt73L-Ozx4efv7w-Pv368wiH2i3UFmylNcyqh4OTxdhlNxFs3mTlZNwzLUBusyTbENBq8XhV8EwweQwW2KfY5pNj0bnKxd3HGNbi9NXfH6Lk3Q3EnT-cR-vr-3ZeLj83l5w-fLs4vG8s4nxtlwdqOs41iEhzwtu-MZNRTSZwwUnZgqW8JVdZb3nnPSS-8N61U3YYRKtgROtv1Tjl9W1yZ9RjKuqWJLi1FgyASOsWFqijsUJtTKdl5PeUwmnyngehVud4p11W5XpXrNfPyqX7ZjK7_l_jruAJ0B5T6FK9c1tdpybF--T-tfwDBmpBb</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>Zhao, Jin-yan</creator><creator>Chen, Zhi-hong</creator><creator>Lin, Wei</creator><creator>Zhong, Xiao-yong</creator><creator>Chen, Xu-zheng</creator><creator>Peng, Jun</creator><creator>Hong, Zhen-feng</creator><general>Chinese Association of Traditional and Western Medicine</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140201</creationdate><title>Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway</title><author>Zhao, Jin-yan ; Chen, Zhi-hong ; Lin, Wei ; Zhong, Xiao-yong ; Chen, Xu-zheng ; Peng, Jun ; Hong, Zhen-feng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-9c1cc843b9371e146d8a732f270e5a7781c2f6029cfc48ff40d5ffa6798b30253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Bile</topic><topic>Carcinoma, Hepatocellular - drug therapy</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Caspases - metabolism</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Shape - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Drugs, Chinese Herbal - pharmacology</topic><topic>Drugs, Chinese Herbal - therapeutic use</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Liver Neoplasms - drug therapy</topic><topic>Liver Neoplasms - pathology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Original Article</topic><topic>Signal Transduction - drug effects</topic><topic>Ursidae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Jin-yan</creatorcontrib><creatorcontrib>Chen, Zhi-hong</creatorcontrib><creatorcontrib>Lin, Wei</creatorcontrib><creatorcontrib>Zhong, Xiao-yong</creatorcontrib><creatorcontrib>Chen, Xu-zheng</creatorcontrib><creatorcontrib>Peng, Jun</creatorcontrib><creatorcontrib>Hong, Zhen-feng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese journal of integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Jin-yan</au><au>Chen, Zhi-hong</au><au>Lin, Wei</au><au>Zhong, Xiao-yong</au><au>Chen, Xu-zheng</au><au>Peng, Jun</au><au>Hong, Zhen-feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway</atitle><jtitle>Chinese journal of integrative medicine</jtitle><stitle>Chin. J. Integr. Med</stitle><addtitle>Chin J Integr Med</addtitle><date>2014-02-01</date><risdate>2014</risdate><volume>20</volume><issue>2</issue><spage>123</spage><epage>129</epage><pages>123-129</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract>Objective
To evaluate the effect of Bear Bile Powder(熊胆粉, BBP) on the growth and apoptosis of HepG2 human hepatocellular carcinoma cells, and investigate the possible molecular mechanisms mediating its anti-cancer activity.
Methods
HepG2 cells were treated with 0.4–1.0 mg/mL of BBP for 24, 48 and 72 h. The viability of HePG2 cells was determined by MTT assay. Cellular morphology was observed via phase-contrast microscopy. Fluorescence-activated cell sorting analysis with Annexin-V/propidium idodide and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazol-carbocyanine iodide (JC-1) staining was performed to determine cell apoptosis and the loss of mitochondrial membrane potential, respectively. Activation of caspase-9 and -3 was evaluated by a colorimetric assay.
Results
The treatment with 0.4–1 mg/mL of BBP for 24, 48, or 72 h respectively reduced cell viability significantly by 7%–60%, 20%–90% or 25%–98%, compared with the untreated control cells (
P
<0.01). In addition, BBP treatment induced morphological changes in HepG2 cells. Furthermore, after treated with 0, 0.4, 0.6, 0.8 and 1.0 mg/mL of BBP, apoptosis cells (including early and late apoptotic cells) were 18.0%±1.3%, 34.9%±2.2%, 33.9%±2.8%, 37.4%±2.8% and 46.0%±2.5%, respectively (
P
<0.05); and the percentage of cells with reduced JC-1 red fluorescence were 6.6%±0.8%, 8.5%±0.8%, 13.5%±1.6%, 17.6%±2.3% and 46.7%±3.6%, respectively (
P
<0.01). Finally, BBP treatment significantly and dose-dependently induced activation of both caspase-9 and caspase-3 in HepG2 cells (
P
<0.05).
Conclusions
BBP could inhibit the growth of HepG2 hepatocellular cancer cells through mitochondrion-mediated apoptosis, which may, in part, explain its anti-cancer activity. BBP may be a potential novel therapeutic agent for the treatment of hepatocellular carcinoma.</abstract><cop>Heidelberg</cop><pub>Chinese Association of Traditional and Western Medicine</pub><pmid>24619237</pmid><doi>10.1007/s11655-013-1581-9</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0415 |
| ispartof | Chinese journal of integrative medicine, 2014-02, Vol.20 (2), p.123-129 |
| issn | 1672-0415 1993-0402 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1507189459 |
| source | Springer Nature |
| subjects | Animals Apoptosis - drug effects Bile Carcinoma, Hepatocellular - drug therapy Carcinoma, Hepatocellular - pathology Caspases - metabolism Cell Proliferation - drug effects Cell Shape - drug effects Cell Survival - drug effects Drugs, Chinese Herbal - pharmacology Drugs, Chinese Herbal - therapeutic use Hep G2 Cells Humans Liver Neoplasms - drug therapy Liver Neoplasms - pathology Medicine Medicine & Public Health Membrane Potential, Mitochondrial - drug effects Mitochondria - drug effects Mitochondria - metabolism Original Article Signal Transduction - drug effects Ursidae |
| title | Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T16%3A25%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bear%20bile%20powder%20(%E7%86%8A%E8%83%86%E7%B2%89)%20induces%20apoptosis%20of%20human%20hepatocellular%20carcinoma%20cells%20via%20mitochondrion-dependent%20pathway&rft.jtitle=Chinese%20journal%20of%20integrative%20medicine&rft.au=Zhao,%20Jin-yan&rft.date=2014-02-01&rft.volume=20&rft.issue=2&rft.spage=123&rft.epage=129&rft.pages=123-129&rft.issn=1672-0415&rft.eissn=1993-0402&rft_id=info:doi/10.1007/s11655-013-1581-9&rft_dat=%3Cproquest_cross%3E1507189459%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c344t-9c1cc843b9371e146d8a732f270e5a7781c2f6029cfc48ff40d5ffa6798b30253%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1507189459&rft_id=info:pmid/24619237&rfr_iscdi=true |