Delphinidin Activates NFAT and Induces IL-2 Production Through SOCE in T Cells

Delphinidin is an anthocyanidin that possesses antioxidant and anti-inflammatory effects; however, some reports suggest that delphinidin has pro-inflammatory properties. For this reason, we assessed the effect of delphinidin on cytokine production in T cells. We demonstrated that delphinidin increas...

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Bibliographic Details
Published in:Cell biochemistry and biophysics 2014-04, Vol.68 (3), p.497-509
Main Authors: Jara, Evelyn, Hidalgo, María A., Hancke, Juan L., Hidalgo, Alejandra I., Brauchi, Sebastian, Nuñez, Luisa, Villalobos, Carlos, Burgos, Rafael A.
Format: Article
Language:English
Subjects:
Anilides - pharmacology
Anthocyanins - pharmacology
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biotechnology
Calcium - metabolism
Cell Biology
Gadolinium
Gadolinium - pharmacology
Humans
Interleukin-2 - biosynthesis
Jurkat Cells
Life Sciences
Lymphocytes
NFATC Transcription Factors - metabolism
Original Paper
Pharmacology/Toxicology
Strontium - metabolism
T-Lymphocytes - drug effects
T-Lymphocytes - metabolism
Thiadiazoles - pharmacology
Translocation
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Summary:Delphinidin is an anthocyanidin that possesses antioxidant and anti-inflammatory effects; however, some reports suggest that delphinidin has pro-inflammatory properties. For this reason, we assessed the effect of delphinidin on cytokine production in T cells. We demonstrated that delphinidin increased the cytosolic-free Ca 2+ concentration by releasing Ca 2+ from intracellular stores and increasing Ca 2+ entry. The putative Ca 2+ release activated Ca 2+ (CRAC) channel inhibitors BTP2 and gadolinium reduced the calcium entry stimulated by the anthocyanidin. Delphinidin induced nuclear factor of activated T cells (NFAT) translocation and NFAT-Luc activity in Jurkat cells and was dependent on the CRAC channel and calcineurin pathway. Delphinidin increased the mRNA expression and production of IL-2 in Jurkat cells and was inhibited by BTP2 and cyclosporine A. Using peripheral blood lymphocytes, we demonstrated that delphinidin increased the production of IL-2 and IFN-γ and was inhibited by BTP2. Taken together, our results suggest that delphinidin exerts immunostimulatory effects on T cells by increasing cytokine production through CRAC channel and NFAT activation.
ISSN:1085-9195
1559-0283
DOI:10.1007/s12013-013-9728-z