Activity of dihydrofolate reductase in the mollicutes Acholeplasma laidlawii and Mycoplasma gallisepticum and their susceptibility to antifolates

Mycoplasma gallisepticum and Acholeplasma laidlawii were found to possess dihydrofolate reductases exhibiting similar specific activities and kinetics, with values in the range of those reported for other microorganisms. The high resistance was found to be due partially to a 130-fold lower affinity...

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Bibliographic Details
Published in:Current microbiology 1988-01, Vol.17 (6), p.351-354
Main Authors: MANDELBAUM-SHAVIT, F, KAHANE, I
Format: Article
Language:English
Subjects:
Acholeplasma laidlawii
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Mycoplasma gallisepticum
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Summary:Mycoplasma gallisepticum and Acholeplasma laidlawii were found to possess dihydrofolate reductases exhibiting similar specific activities and kinetics, with values in the range of those reported for other microorganisms. The high resistance was found to be due partially to a 130-fold lower affinity of the target enzyme for this antifolate, but another mechanism, presumably impaired transport, must also be involved. This is the first report of dihydrofolate reductase activity in Mollicutes.
ISSN:0343-8651
1432-0991
DOI:10.1007/BF01570877