Diagnostic screening method for the determination of trichothecene exposure in animals

A rapid screening method for diagnosis of exposure of the trichothecene mycotoxins T-2, diacetoxyscirpenol (DA), deoxynivalenol (DON), and nivalenol (NIV) has been developed. The toxins are extracted from urine and plasma with a C-18 cartridge followed by a Florisil minicolumn cleanup. The residues...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1988-01, Vol.36 (1), p.74-79
Main Authors: Rood, Harold D, Buck, William B, Swanson, Steven P
Format: Article
Language:English
Subjects:
ALIMENT POUR ANIMAUX
BETAIL
Biological and medical sciences
DEGRADATION
DETERIORO
FEEDS
FUSARIUM
GANADO
LIVESTOCK
Medical sciences
MICOTOXINAS
MYCOTOXINE
MYCOTOXINS
PIENSOS
Plant poisons toxicology
Toxicology
TRICHOTHECENE
TRICHOTHECENES
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Summary:A rapid screening method for diagnosis of exposure of the trichothecene mycotoxins T-2, diacetoxyscirpenol (DA), deoxynivalenol (DON), and nivalenol (NIV) has been developed. The toxins are extracted from urine and plasma with a C-18 cartridge followed by a Florisil minicolumn cleanup. The residues are then hydrolyzed with NaOH to form the parent alcohols of any esterified toxins, with a final cleanup using a silica cartridge. Quantitation of the trifluoroacyl derivatives of the parent alcohols T-2 tetraol (TOL), scirpentriol (STR), DON, and NIV is by GLC/ECD with a detection limit of better than 25 ng/mL. Mass spectral confirmation by negative chemical ionization is possible with little or no sample workup.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00079a019