Formation of triple-helical nucleic acids studied by using antibodies specific for poly(A) multiplied by poly(U) multiplied by poly(U)

The formation of the triple helix of poly(A) multiplied by poly(U) multiplied by poly(U) was studied by using antibodies specific to poly(A) multiplied by poly(U) multiplied by poly(U). The 10-11 base chain length for oligo(A) and the 20-30 base chain length for oligo(U) may be the minimum sizes req...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1987-01, Vol.102 (5), p.1203-1212
Main Authors: Kitagawa, Y, Okuhara, E
Format: Article
Language:English
Online Access:Get full text
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Summary:The formation of the triple helix of poly(A) multiplied by poly(U) multiplied by poly(U) was studied by using antibodies specific to poly(A) multiplied by poly(U) multiplied by poly(U). The 10-11 base chain length for oligo(A) and the 20-30 base chain length for oligo(U) may be the minimum sizes required to maintain a stable triple helix. Double-stranded poly(A) multiplied by poly(U) which was the core of triple-stranded poly(A) multiplied by poly(U) multiplied by poly(U) could bind poly(U) and produce an analogue of poly(A) multiplied by poly(U) multiplied by poly(U) reactive with the antibodies even if the poly(A) or poly(U) was brominated or acetylated to the extent of 35-55%. However, brominated or acetylated poly(U) did not produce a stable triple helix with double-stranded poly(A) multiplied by poly(U).
ISSN:0021-924X