Laminin-5 gamma 2 chain expression is associated with intensity of tumor budding and density of stromal myofibroblasts in oral squamous cell carcinoma

Background Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers worldwide. Laminin‐5 gamma 2 chain (laminin‐5 γ2) is a protein associated to a migratory phenotype in epithelial neoplastic cells. Stromal myofibroblasts also play a significant role in tumor invasion, due to its abi...

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Published in:Journal of oral pathology & medicine 2014-03, Vol.43 (3), p.199-204
Main Authors: Marangon Junior, Helvécio, Rocha, Valéria Nazaré, Leite, Camila Ferreira, de Aguiar, Maria Cássia Ferreira, Souza, Paulo Eduardo Alencar, Horta, Martinho Campolina Rebello
Format: Article
Language:English
Subjects:
Actins - analysis
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Carcinoma, Squamous Cell - chemistry
Carcinoma, Squamous Cell - pathology
Cell Count
Dentistry
Female
Humans
Immunohistochemistry
Keratins - analysis
Laminin - analysis
laminin-5 γ2
Male
Medical sciences
Middle Aged
Mouth Neoplasms - chemistry
Mouth Neoplasms - pathology
myofibroblast
Myofibroblasts - chemistry
Myofibroblasts - pathology
Neoplasm Invasiveness
oral squamous cell carcinoma
Otorhinolaryngology. Stomatology
tumor budding
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
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Summary:Background Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers worldwide. Laminin‐5 gamma 2 chain (laminin‐5 γ2) is a protein associated to a migratory phenotype in epithelial neoplastic cells. Stromal myofibroblasts also play a significant role in tumor invasion, due to its ability to modify the extracellular matrix. Tumor budding is a morphologic marker of tumor invasion. The aim of this study was to evaluate the expression of laminin‐5 γ2 in OSCC and its association with intensity of tumor budding and density of stromal myofibroblasts. Methods Paraffin‐embedded archival samples of 57 OSCC patients were evaluated. Immunohistochemistry was employed to detect laminin‐5 γ2, alpha smooth muscle actin (marker of stromal myofibroblasts), and multicytokeratin (to identify OSCC cells in tumor budding evaluation). Laminin‐5 γ2 expression and its association with intensity of tumor budding and density of stromal myofibroblasts were analyzed. Association among intensity of tumor budding and density of stromal myofibroblasts was also evaluated. Results Higher laminin‐5 γ2 expression was associated with high‐intensity tumor budding (P 
ISSN:0904-2512
1600-0714
DOI:10.1111/jop.12121