NUCB2/nesfatin-1: A new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties, an in vitro study

ABSTRACT Nesfatin‐1 is a recently discovered satiety‐inducing adipokine identified in hypothalamic regions that regulates energy balance. So far, no data exist on NUCB2/nesfatin‐1 localization in human and murine chondrocytes. Here, we therefore investigated NUCB2/nesfatin‐1 gene and protein express...

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Published in:Journal of orthopaedic research 2014-05, Vol.32 (5), p.653-660
Main Authors: Scotece, Morena, Conde, Javier, Abella, Vanessa, López, Veronica, Lago, Francisca, Pino, Jesús, Gómez-Reino, Juan J., Gualillo, Oreste
Format: Article
Language:English
Subjects:
Animals
Calcium-Binding Proteins - biosynthesis
Cell Differentiation
Cell Line
Cells, Cultured
Chemokine CCL3 - biosynthesis
chondrocytes
Chondrocytes - metabolism
Cytokines
DNA-Binding Proteins - biosynthesis
Humans
inflammation
Interleukin-6 - biosynthesis
Interleukin-8 - biosynthesis
Mice
Nerve Tissue Proteins - biosynthesis
Nesfatin-1
NUCB2
RNA, Messenger - metabolism
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Summary:ABSTRACT Nesfatin‐1 is a recently discovered satiety‐inducing adipokine identified in hypothalamic regions that regulates energy balance. So far, no data exist on NUCB2/nesfatin‐1 localization in human and murine chondrocytes. Here, we therefore investigated NUCB2/nesfatin‐1 gene and protein expression in human and murine chondrocytes and the effect of nesfatin‐1 on pro‐inflammatory cytokines expression. Peptide localization was performed by laser confocal microscopy, NUCB2 mRNA expression was studied by RT‐PCR and protein secretion was measured by XMap technology and Western blot analysis. First, we demonstrated cytoplasmic localization of NUCB2/nesfatin‐1 peptide in both human and murine chondrocytes. We present evidence that both mRNA and protein expression of NUCB2 were increased during the differentiation of ATDC5 murine chondrocyte cell line. Furthermore, we demonstrated that nesfatin‐1 induces IL‐6 and MIP‐1α mRNA expression and protein secretion in ATDC‐5 cells challenged with IL‐1, and also increases COX‐2 mRNA expression in these cells. Finally, nesfatin‐1 provoked a clear induction of pro‐inflammatory agents, such as COX‐2, IL‐8, IL‐6, and MIP‐1α in human primary chondrocytes from OA patients. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:653–660, 2014.
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.22585