Modulation of murine T and B cell reactivity after short-term cadmium exposure in vivo

This study assessed early effects of short-term Cd exposure on T and B cell responsiveness. Spleen cells from mice injected s.c. with a daily dosage of 1 mg, 0.33 mg, or 0.11 mg Cd (as CdCl2) per kg body weight for 5 days were examined for their potential to generate alloreactive T cells in a mixed...

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Bibliographic Details
Published in:Archives of toxicology 1988-08, Vol.62 (1), p.22-28
Main Authors: HURTENBACH, U, OBERBARNSCHEIDT, J, GLEICHMANN, E
Format: Article
Language:English
Subjects:
Animals
B-Lymphocytes - drug effects
Biological and medical sciences
Cadmium - administration & dosage
Cadmium - toxicity
Cell Count
Cells, Cultured
Chemical and industrial products toxicology. Toxic occupational diseases
Medical sciences
Metals and various inorganic compounds
Mice
Mice, Inbred Strains
Spleen - cytology
Spleen - drug effects
T-Lymphocytes - drug effects
Time Factors
Toxicology
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Summary:This study assessed early effects of short-term Cd exposure on T and B cell responsiveness. Spleen cells from mice injected s.c. with a daily dosage of 1 mg, 0.33 mg, or 0.11 mg Cd (as CdCl2) per kg body weight for 5 days were examined for their potential to generate alloreactive T cells in a mixed lymphocyte reaction (MLR) and for mitogen reactivity to concanavalin A (Con A) in vitro. Spleen cells from the same mice were also assayed for the total number of IgM- and IgG-secreting B cells. Whereas alloreactivity was reduced, mitogen response to Con A was not different from controls or was even enhanced. The decrease in allogeneic MLR was dependent on the injected Cd dosage. No difference in susceptibility to Cd-induced effects was observed among the mouse strains tested, i.e. BALB/c, DBA/2, C57BL/6, and C3H/He. Co-cultivation of spleen cells, obtained from Cd-treated mice that exhibited deficient T cell reactivity, with splenic responder cells from untreated mice resulted in dose-dependent suppression of the normal MLR. These results indicate that the harmful effects of Cd on the immune system include the inhibition of antigen-specific T cell responses by the activation of an antigen non-specific suppressor system. In contrast to the suppressed allogeneic MLR, the same spleen cell populations showed augmented numbers of IgM- and IgG-antibody producing cells.
ISSN:0340-5761
1432-0738
DOI:10.1007/BF00316252