Morphologic study of the liver of lambari (Astyanax altiparanae) with emphasis on the distribution of cytokeratin

• Published in: Fish physiology and biochemistry 2014-04, Vol.40 (2), p.571-576
• Main Authors: Chehade, Chayrra, Cassel, Mônica, Borella, Maria Inês, Costa, Fabiano Gonçalves
• Format: Article
• Language: English
• Subjects: adults; Alcohol; Andira; Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Astyanax altiparanae; Bile ducts; Biomedical and Life Sciences; blood flow; Brazil; Characidae - anatomy & histology; Characidae - metabolism; Connective tissue; Cytokeratin; endothelial cells; Fish Proteins - metabolism; fluorescence; Freshwater & Marine Ecology; hepatocytes; Hepatocytes - cytology; Hepatocytes - metabolism; Hepatopancreas - anatomy & histology; Hepatopancreas - metabolism; Histology; Immunohistochemistry; Keratins - metabolism; Kupffer Cells - cytology; Kupffer Cells - metabolism; Life Sciences; Liver; Liver - anatomy & histology; Liver - blood supply; Liver - metabolism; Morphology; Physiology; Teleostei; Tissue Distribution; Veins & arteries; Zoology
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-013-9867-6

Studies on the morphology of the liver of teleosts reflect some controversy in the interpretation of the data, but also provide confirmation of variations in the structure of the organ in several species. Thus, we intend to understand the specific structural organization of the liver of Astyanax altiparanae. Specimens were collected in the city of Andirá, Paraná, Brazil. The livers were processed according to histological routine for inclusion in Paraplast, and the sections were stained with HE and Mallory’s trichrome or followed the protocol for fluorescence immunohistochemistry, anti-cytokeratin. The liver of A. altiparanae was covered by a capsule of connective tissue, without delimiting lobes. The hepatocytes had an arrangement in cords around sinusoids. Melanomacrophage centers were observed. The vascular components and intrahepatic pancreatic acini were distributed between hepatocytes. Presence of cytokeratin was detected in tissues that lined the liver and endothelial cells of sinusoids. The comparison of the liver of A. altiparanae to other characids corroborates with the fact that there is variation in the morphology of the liver even between closely related species. Moreover, it appears that in this species, endothelial cells of sinusoids can synthesize the cytokeratin filaments required for the regulation of blood flow in capillaries in adults.