Regulation of the binding of the BMP-2 growth factor with collagen by blood plasma fibronectin

The release kinetics of recombinant human bone morphogenic factor 2 (rhBMP-2) from collagen hydrogel in the presence of human blood plasma have been studied. The expulsion of rhBMP-2 from the collagen-BMP-2 complex by the competitive adhesion of collagen-binding proteins penetrating from plasma was...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2014-03, Vol.50 (2), p.200-205
Main Authors: Osidak, E. O, Osidak, M. S, Sivogrivov, D. E, Portnaya, T. S, Grunina, T. M, Soboleva, L. A, Lunin, V. G, Karyagina, A. S, Domogatskii, S. P
Format: Article
Language:English
Subjects:
adhesion
adverse effects
Biochemistry
Biomedical and Life Sciences
blood plasma
Collagen
fibronectins
humans
hydrocolloids
Hydrogels
Kinetics
Life Sciences
Medical Microbiology
Microbiology
Plasma
Proteins
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Summary:The release kinetics of recombinant human bone morphogenic factor 2 (rhBMP-2) from collagen hydrogel in the presence of human blood plasma have been studied. The expulsion of rhBMP-2 from the collagen-BMP-2 complex by the competitive adhesion of collagen-binding proteins penetrating from plasma was firstly recognized. It was experimentally proven that that blood plasma fibronectin is the main collagen-binding protein, which is responsible for the controlled release of rhBMP-2. As a result, a new collagen hydrogel with the incorporation of fibronectin was created which retained rhBMP-2 for a twice longer period as compared to the ordinary collagen hydrogel. A distinctive feature of this new collagen-fibronectin matrix is the slow release of rhBMP-2 in the first three days which allows for the avoiding of adverse effects in clinics caused by the rapid release of large amounts of rhBMP-2 from collagen hydrogel.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683814020148