Antioxidant activity of polysaccharide extracted from Pleurotus eryngii using response surface methodology

Ultrasonic technology was applied for polysaccharide extraction from the fruiting bodies of P. Eryngii, and response surface methodology (RSM) was used to optimize the effects of processing parameters on polysaccharide extraction yield. Three independent variables were extraction time (A), ultrasoni...

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Bibliographic Details
Published in:International journal of biological macromolecules 2014-04, Vol.65, p.28-32
Main Authors: Zhang, Anqiang, Li, Xueqing, Xing, Chen, Yang, Junhong, Sun, Peilong
Format: Article
Language:English
Subjects:
Antioxidant activity
Biphenyl Compounds - chemistry
Free Radical Scavengers - chemistry
Free Radical Scavengers - isolation & purification
Free Radical Scavengers - pharmacology
Oxidation-Reduction - drug effects
Picrates - chemistry
Pleurotus - chemistry
Pleurotus eryngii
Polysaccharide
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Reactive Oxygen Species - chemistry
Response surface methodology (RSM)
Time Factors
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Summary:Ultrasonic technology was applied for polysaccharide extraction from the fruiting bodies of P. Eryngii, and response surface methodology (RSM) was used to optimize the effects of processing parameters on polysaccharide extraction yield. Three independent variables were extraction time (A), ultrasonic power (B) and the ratio of solvent to sample (C), respectively. Results showed that the maximum yield of P. eryngii polysaccharide (PEPS) was obtained at an optimum condition: extraction time 39min, ultrasonic power 517W, the ratio of water to material 19mL/g, and the PEPS extracting yield reached 34.3% under the conditions. PEPS were precipitated into three crude polysaccharides, PEPS30, PEPS60 and PEPS80, by different concentrations of ethanol respectively. The antioxidant activities of these three polysaccharides were evaluated. The results showed that PEPS80 had the best reducing power, DPPH radical scavenging ability and oxygen radical scavenging ability followed by PEPS60 and PEPS30.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2014.01.013