4,4'-Diisothiocyanato-stilbene-2,2'-disulfonic acid inhibits CD3-T cell antigen receptor-stimulated Ca super(2+) influx in human T lymphocytes

Stimulation of the CD3-T cell antigen receptor complex on T lymphocytes results in a rapid rise in intracellular calcium from both intra- and extracellular sources. The former is thought to be released from the endoplasmic reticulum in response to inositol trisphosphate, while the latter enters the...

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Bibliographic Details
Published in:The Journal of biological chemistry 1988-01, Vol.263 (36), p.19535-19540
Main Authors: Rosoff, P M, Hall, C, Gramates, L S, Terlecky, SR
Format: Article
Language:English
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Summary:Stimulation of the CD3-T cell antigen receptor complex on T lymphocytes results in a rapid rise in intracellular calcium from both intra- and extracellular sources. The former is thought to be released from the endoplasmic reticulum in response to inositol trisphosphate, while the latter enters the cells through a membrane potential-sensitive transporter. In this report the authors show that the stilbene disulfonate, DIDS (4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid), inhibited the ability of monoclonal anti-CD3 complex antibodies to stimulate an influx of calcium in the human T lymphocyte cell line, Jurkat. The data suggest that the function of the CD3-T cell receptor-activated calcium channel/transporter may be dependent on or regulated by extracellular Cl super(-).
ISSN:0021-9258