Expression level of sonic hedgehog correlated with the speed of gastric mucosa regeneration in artificial gastric ulcers

• Published in: Journal of gastroenterology and hepatology 2014-04, Vol.29 (4), p.736-741
• Main Authors: Tanaka, Takeshi, Arai, Makoto, Minemura, Shoko, Oyamada, Arata, Saito, Keiko, Jiang, Xia, Tsuboi, Masaru, Sazuka, Sayuri, Maruoka, Daisuke, Matsumura, Tomoaki, Nakagawa, Tomoo, Sugaya, Shigeru, Kanda, Tatsuo, Katsuno, Tatsuro, Kita, Kazuko, Kishimoto, Takashi, Imazeki, Fumio, Kaneda, Atsushi, Yokosuka, Osamu
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Female; Gastric Mucosa - physiology; gastric mucosa regeneration; Gene Expression Regulation, Developmental - genetics; Hedgehog Proteins - genetics; Hedgehog Proteins - physiology; Humans; IL1β; Interleukin-1beta - genetics; Interleukin-1beta - physiology; Male; Middle Aged; Multivariate Analysis; Regeneration - genetics; RNA, Messenger - analysis; Signal Transduction; sonic hedgehog; Stomach Ulcer - physiopathology; Time Factors; Wound Healing - genetics; Wound Healing - physiology
• ISSN: 0815-9319; 1440-1746
• DOI: 10.1111/jgh.12445

Background and Aim Gastric ulcer healing is a complex process involving cell proliferation and tissue remodeling. Sonic hedgehog (Shh) activates the Shh signaling pathway, which plays a key role in processes such as tissue repair. Shh and interleukin 1β (IL1β) have been reported to influence the proliferation of gastric mucosa. We evaluated the relationships between the speed of gastric ulcer healing and the levels of expression of Shh and IL1β. Methods The study included 45 patients (mean age 71.9 ± 9.0 years; M/F, 30/15) who underwent endoscopic submucosal dissection (ESD) for gastric cancer, followed by standard dose of oral proton‐pump inhibitor for 4 weeks. Subsequently, the size of ESD‐induced artificial ulcers were measured to determine the speed of gastric ulcer healing, and regenerating mucosa around the ulcers and appropriately matched controls were collected from patients by endoscopic biopsy. Polymerase chain reaction (PCR) array analysis of genes in the Shh signaling pathway was performed, and quantitative reverse transcription (RT)‐PCR was used to measure IL1β mRNA. Results The levels of Shh and IL1β mRNA were 3.0 ± 2.7‐fold and 2.5 ± 2.5‐fold higher, respectively, in regenerating mucosa of artificial ulcers than in appropriately matched controls, with the two being positively correlated (r = 0.9, P