The Use of TLC-DPPH• Test with Image Processing to Study Direct Antioxidant Activity of Phenolic Acid Fractions of Selected Lamiaceae Family Species

TLC coupled with 2,2-diphenyl-1-picrylhydrazyl staining was used to analyze phenolic acid fractions of selected Salvia and Thymus species. Documented videoscans were processed by means of an image processing program. This is the first time that free phenolic acids fractions, as well as fractions con...

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Bibliographic Details
Published in:Journal of AOAC International 2013-11, Vol.96 (6), p.1228-1232
Main Authors: CIESLA, Lukasz, STASZEK, Dorota, KOWALSKA, Teresa, WAKSMUNDZKA-HAJNOS, Monika
Format: Article
Language:English
Subjects:
Antioxidants - chemistry
Biological and medical sciences
Biphenyl Compounds - analysis
Caffeic Acids - chemistry
Chromatography, Thin Layer - methods
Free Radical Scavengers - chemistry
Free Radicals - chemistry
Fundamental and applied biological sciences. Psychology
Hydrolysis
Hydroxybenzoates - chemistry
Lamiaceae - chemistry
Methanol
Phenol
Picrates - analysis
Plant Extracts - chemistry
Salvia - chemistry
Signal Processing, Computer-Assisted
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Summary:TLC coupled with 2,2-diphenyl-1-picrylhydrazyl staining was used to analyze phenolic acid fractions of selected Salvia and Thymus species. Documented videoscans were processed by means of an image processing program. This is the first time that free phenolic acids fractions, as well as fractions containing phenolic acids derived from basic and acidic hydrolysis, have been analyzed and compared for selected sage and thyme species. The analyzed samples along with caffeic acid (CA; standard) were chromatographed on silica gel plates with toluene-ethyl acetate-formic acid (60 + 40 + 1, v/v/v) mobile phase. The extracts were investigated with respect to the activity of CA. It was found that CA was most abundant in the fractions derived from basic hydrolysis. This compound was not detected in any of the fractions obtained after acidic hydrolysis. S. officinalis and S. triloba have similar free radical scavenging activity fingerprints obtained for all the analyzed fractions.
ISSN:1060-3271
1944-7922
DOI:10.5740/jaoacint.SGECiesla