Improving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction site

Here we demonstrate that elimination of ClaI restriction site from the sequence of a plasmid DNA increases the efficiency of transformation of Shewanella oneidensis MR-1 significantly. To achieve reliable transformation of S. oneidensis MR-1 plasmids either lacking ClaI site or isolated from primary...

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Bibliographic Details
Published in:Journal of microbiological methods 2014-04, Vol.99, p.35-37
Main Authors: Rachkevych, Nazarii, Sybirna, Kateryna, Boyko, Solomiya, Boretsky, Yuriy, Sibirny, Andriy
Format: Article
Language:English
Subjects:
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA restriction–modification
DNA, Bacterial - genetics
DNA, Bacterial - metabolism
Plasmids
Sequence Deletion
Shewanella - enzymology
Shewanella - genetics
Shewanella oneidensis
Transformation
Transformation, Bacterial
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Summary:Here we demonstrate that elimination of ClaI restriction site from the sequence of a plasmid DNA increases the efficiency of transformation of Shewanella oneidensis MR-1 significantly. To achieve reliable transformation of S. oneidensis MR-1 plasmids either lacking ClaI site or isolated from primary transformants of S. oneidensis should be used. •Transformation efficiency of S. oneidensis is influenced by presence of ClaI site in the plasmid.•Elimination of ClaI site significantly improves transformation efficiency of S. oneidensis.•Apparently DNA restriction-modification systems are very active in S. oneidensis MR-1.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2014.01.009