Effect of the Oral Intake of Probiotic Pediococcus acidilactici BA28 on Helicobacter pylori Causing Peptic Ulcer in C57BL/6 Mice Models

• Published in: Applied biochemistry and biotechnology 2014, Vol.172 (2), p.973-983
• Main Authors: Kaur, Baljinder, Garg, Neena, Sachdev, Atul, Kumar, Balvir
• Format: Article
• Language: English
• Subjects: Administration, Oral; animal models; Animals; Bacteria; Bacterial infections; Bacteriocins - pharmacology; Biochemistry; Biological and medical sciences; biopsy; Biotechnology; Chemistry; Chemistry and Materials Science; Colony Count, Microbial; diet; Disease Models, Animal; feces; Feces - microbiology; Female; Fundamental and applied biological sciences. Psychology; gastric mucosa; gastritis; growth retardation; Helicobacter pylori; Helicobacter pylori - growth & development; Helicobacter pylori - physiology; Humans; lactic acid bacteria; Medical instruments; Mice; Mice, Inbred C57BL; Microbial Sensitivity Tests; oral administration; Pediococcus - chemistry; Pediococcus acidilactici; Peptic Ulcer - drug therapy; Peptic Ulcer - microbiology; Peptic Ulcer - pathology; peptic ulcers; polyethylene; Probiotics; Probiotics - administration & dosage; Probiotics - pharmacology; Probiotics - therapeutic use; Rodents; Stomach - drug effects; Stomach - microbiology; Stomach - pathology; Ulcers
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-013-0585-4

Probiotic lactic acid bacteria are being proposed to cure peptic ulcers by reducing colonization of Helicobacter pylori within the stomach mucosa and by eradicating already established infection. In lieu of that, in vitro inhibitory activity of pediocin-producing probiotic Pediococcus acidilactici BA28 was evaluated against H. pylori by growth inhibition assays. Further, chronic gastritis was first induced in two groups of C57BL/6 mice by orogastric inoculation with H. pylori with polyethylene catheter, and probiotic P. acidilactici BA28 was orally administered to study the eradication and cure of peptic ulcer disease. H. pylori and P. acidilactici BA28 were detected in gastric biopsy and fecal samples of mice, respectively. A probiotic treatment with P. acidilactici BA28, which is able to eliminate H. pylori infection and could reverse peptic ulcer disease, is being suggested as a co-adjustment with conventional antibiotic treatment. The study provided an evidence of controlling peptic ulcer disease, by diet modulation.