A disintegrin and metalloprotease-12 is type I myofiber specific in Bos taurus and Bos indicus cattle

A disintegrin and metalloproteinase-12 (ADAM12) is involved in the regulation of myogenesis and adipogenesis and is of interest as a potential target to manipulate skeletal muscle development and intramuscular fat (IMF) deposition in cattle to increase beef yield and improve meat quality. The longis...

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Bibliographic Details
Published in:Journal of animal science 2014-04, Vol.92 (4), p.1473-1483
Main Authors: Coles, C A, Wadeson, J, Knight, M I, Cafe, L M, Johns, W H, White, J D, Greenwood, P L, McDonagh, M B
Format: Article
Language:English
Subjects:
ADAM Proteins - genetics
ADAM Proteins - metabolism
ADAM12 Protein
Animals
Blotting, Western
Cattle - physiology
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation, Enzymologic - physiology
Male
Membrane Proteins - genetics
Membrane Proteins - metabolism
Muscle Fibers, Slow-Twitch - enzymology
Muscle Fibers, Slow-Twitch - metabolism
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Summary:A disintegrin and metalloproteinase-12 (ADAM12) is involved in the regulation of myogenesis and adipogenesis and is of interest as a potential target to manipulate skeletal muscle development and intramuscular fat (IMF) deposition in cattle to increase beef yield and improve meat quality. The longissimus thoracis muscle (LM) and semitendinosus muscle (STM) from 5 Bos taurus (Angus) and 5 Bos indicus (Brahman) cattle were collected for histological and ADAM12 gene and protein expression analysis. Myofiber typing was used to determine if ADAM12 expression patterns related to differences in muscling and IMF deposition, which are influenced by proportions of the different myofiber types. The STM was found to contain a higher proportion of glycolytic myofibers than the LM, which contained a greater proportion of oxidative myofibers (myofiber ratio of glycolytic to more oxidative types in LM and STM of 1.1 and 3.5, respectively; P < 0.05). ADAM12 gene expression, fluorescent immunohistochemical staining for ADAM12, and image analysis found ADAM12 to be greater in the LM (P < 0.05). Regression analysis found a strong, positive relationship for the distribution of ADAM12 against the proportion of type I myofibers (P < 0.05, r(2) = 0.86). These findings suggest ADAM12 is upregulated in muscles with more slow-oxidative myofibres, such as the LM, and is linked to type I myofibers in cattle. ADAM12 may be important in the regulation and maintenance slow myofibers in the LM muscle.
ISSN:1525-3163
DOI:10.2527/jas.2013-7069