Green tea (−)-epigallocatechin gallate suppresses IGF-I and IGF-II stimulation of 3T3-L1 adipocyte glucose uptake via the glucose transporter 4, but not glucose transporter 1 pathway

• Published in: General and comparative endocrinology 2014-04, Vol.199, p.46-55
• Main Authors: Ku, Hui-Chen, Tsuei, Yi-Wei, Kao, Chung-Cheng, Weng, Jueng-Tsueng, Shih, Li-Jane, Chang, Hsin-Huei, Liu, Chi-Wei, Tsai, Shu-Wei, Kuo, Yow-Chii, Kao, Yung-Hsi
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; Acetylcysteine - pharmacology; Adipocyte glucose uptake; Adipocytes - drug effects; Adipocytes - metabolism; Animals; Antibodies - metabolism; Catechin - analogs & derivatives; Catechin - pharmacology; Cell Membrane - drug effects; Cell Membrane - metabolism; Cytoplasm - drug effects; Cytoplasm - metabolism; Endocrinology & Metabolism; Epigallocatechin gallate; Glucose - metabolism; Glucose transporter; Glucose Transporter Type 1 - metabolism; Glucose Transporter Type 4 - metabolism; Green tea; Insulin-like growth factor; Insulin-Like Growth Factor I - metabolism; Insulin-Like Growth Factor II - metabolism; Mice; Phosphorylation - drug effects; Protein Transport; Receptors, Laminin - metabolism; Signal Transduction - drug effects; Tea - chemistry
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2014.01.008

Highlights • EGCG suppresses IGF stimulation of adipocyte glucose uptake. • EGCG inhibits IGF-increased levels of GLUT4 translocation from the cytosol to the plasma membrane. • NAC does not block the anti-IGF effect of EGCG on adipocyte glucose uptake. • EGCG inhibits the IGF-stimulated phosphorylation of IGF signaling molecules, PKCζ/λ. • The 67LR mediates the anti-IGF effect of EGCG on adipocyte glucose uptake.