NtcA is responsible for accumulation of the small isoform of ferredoxin:NADP oxidoreductase

In several cyanobacteria, petH, the gene encoding ferredoxin:NADP oxidoreductase (FNR), is transcribed from at least two promoters depending on growth conditions. Two transcripts (short and long) are translated from two different translation initiation sites, resulting in two isoforms (large and sma...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2014-04, Vol.160 (Pt 4), p.789-794
Main Authors: Omairi-Nasser, Amin, Galmozzi, Carla V, Latifi, Amel, Muro-Pastor, M Isabel, Ajlani, Ghada
Format: Article
Language:English
Subjects:
Bacterial Proteins - metabolism
Binding Sites
DNA Mutational Analysis
DNA-Binding Proteins - metabolism
Ferredoxin-NADP Reductase - metabolism
Flavoproteins - metabolism
Gene Expression
Gene Expression Regulation, Bacterial
Promoter Regions, Genetic
Protein Isoforms - metabolism
Synechocystis - enzymology
Synechocystis - genetics
Transcription Factors - metabolism
Transcription, Genetic
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Summary:In several cyanobacteria, petH, the gene encoding ferredoxin:NADP oxidoreductase (FNR), is transcribed from at least two promoters depending on growth conditions. Two transcripts (short and long) are translated from two different translation initiation sites, resulting in two isoforms (large and small, respectively). Here, we show that in Synechocystis PCC6803 the global transcriptional regulator NtcA activates transcription from the distal petH promoter. Modification of the NtcA-binding site prevents NtcA binding to the promoter in vitro and abolishes accumulation of the small isoform of FNR in vivo. We also demonstrate that a similar petH transcription and translation regime occurs in other cyanobacteria. The conditions under which this system operates provide hints for the function of each FNR isoform.
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.076042-0