In vitro and in vivo anti-cancer activity of formononetin on human cervical cancer cell line HeLa

Worldwide, cervical cancer (CC) is the third most common malignancy in women, and it remains a leading cause of cancer-related death of women. Genomic studies indicate that phosphoinositide 3-kinase (PI3K)/AKT signaling is one of the most frequently deregulated pathways in several human cancers, inc...

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Bibliographic Details
Published in:Tumor biology 2014-03, Vol.35 (3), p.2279-2284
Main Authors: Jin, Yue-mei, Xu, Tian-min, Zhao, Yan-hui, Wang, Yi-chao, Cui, Man-hua
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents - pharmacology
Apoptosis - drug effects
Biomedical and Life Sciences
Biomedicine
Blotting, Western
Cancer Research
Cell Proliferation - drug effects
Cellular biology
Cervical cancer
Estrogens
Female
Flow Cytometry
HeLa Cells
Humans
Isoflavones - pharmacology
Kinases
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasms, Experimental - drug therapy
Phosphatidylinositol 3-Kinases - metabolism
Phytochemicals
Phytoestrogens - pharmacology
Proto-Oncogene Proteins c-akt - metabolism
Research Article
Signal Transduction - drug effects
Uterine Cervical Neoplasms - metabolism
Xenograft Model Antitumor Assays
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Summary:Worldwide, cervical cancer (CC) is the third most common malignancy in women, and it remains a leading cause of cancer-related death of women. Genomic studies indicate that phosphoinositide 3-kinase (PI3K)/AKT signaling is one of the most frequently deregulated pathways in several human cancers, including CC. This signaling pathway has an important role in cancer cell proliferation, survival, motility, and metabolism, and therefore could be an attractive therapeutic target. In a previous study, we used a sensitive and high-speed homogeneous assay for the detection of kinase activity and for screening of PI3K/AKT signaling inhibitors in a high-throughput screening (HTS) format and then obtain formononetin, as an O -methylated isoflavone existed in a number of plants and herbs like Astragalus membranaceus . We showed that formononetin inhibited the phosphorylation of AKT and induced the apoptosis of CC cell line HeLa in a dose-dependent manner. Furthermore, formononetin suppressed xenograft tumor growth in nude mice. Our results indicated that formononetin may be used as an anti-cancer drug for cervical cancer in the future.
ISSN:1010-4283
1423-0380
DOI:10.1007/s13277-013-1302-1