Remote ischemic conditioning enhanced the early recovery of renal function in recipients after kidney transplantation: a randomized controlled trial

• Published in: The Journal of surgical research 2014-05, Vol.188 (1), p.303-308
• Main Authors: Wu, Jianyong, MD, Feng, Xiaoxiao, MD, Huang, Hongfeng, MD, Shou, Zhangfei, MD, Zhang, Xiaohui, MD, Wang, Rending, MD, Chen, Yanyan, MD, Chen, Jianghua, MD
• Format: Article
• Language: English
• Subjects: Acute Kidney Injury - prevention & control; Adult; Donation after cardiac death; eGFR; Female; Humans; Ischemic Preconditioning; Ischemic reperfusion injury; Kidney - physiology; Kidney Function Tests; Kidney Transplantation; Lower Extremity - blood supply; Male; Middle Aged; Recovery of Function; Remote ischemic conditioning; Reperfusion Injury - prevention & control; Surgery; Young Adult
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2013.06.058

Abstract Background To investigate whether remote ischemic conditioning (RIC) can attenuate ischemic reperfusion injury (IRI) in recipients after kidney transplantation using donation after cardiac death. Methods Forty-eight recipients referred for kidney transplantation were recruited. The paired recipients who received the kidneys from the same donor were randomly assigned (one received RIC and the other did not). RIC was induced by three 5-min cycles of brief repetitive ischemia and reperfusion by clamping the exposed external iliac artery. Blood samples were withdrawn at hour 2, hour 12, days 1–7, day 14, and day 30 to measure serum creatinine level and estimated glomerular filtration rate after transplantation. Urine samples were collected at hours 2, 12, 24, and 48 to measure urine neutrophil gelatinase–associated lipocalin after transplantation. Renal tissues were obtained at 30 min for histologic changes after transplantation. Results There were no significant differences in clinical characteristics of the recipients and donors between RIC and control groups. The serum creatinine level was lower in the RIC group compared with that of the control group (12 h, days 1–14, P < 0.05; other P  > 0.05); the estimated glomerular filtration rate was higher in the RIC group compared with that of the control group (12 h, days 1–14, P < 0.05; other P > 0.05); urine neutrophil gelatinase–associated lipocalin, an early marker of IRI, was lower in the RIC group at hours 2, 12, 24, and 48 (2 h, 48 h, P > 0.05; 12 h, 24 h, P < 0.05) compared with that of the control group. The graft pathology showed no differences between RIC and control groups. Conclusions RIC enhanced the early recovery of renal function in recipients after kidney transplantation. Our results provide a novel potential approach to attenuate transplantation-associated IRI.