Cytokinins in Azotobacter vinelandii culture medium

Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were det...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1989-01, Vol.55 (1), p.266-267
Main Authors: Taller, B.J, Wong, T.Y
Format: Article
Language:English
Subjects:
Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents
Applied microbiology
AZOTOBACTER
Azotobacter vinelandii
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
BIOSINTESIS
BIOSYNTHESE
Biotechnology
CITOCININAS
CYTOKININE
Fundamental and applied biological sciences. Psychology
MEDIO DE CULTIVO
Microbiology
Microorganism-Plant Interactions
MILIEU DE CULTURE
Mission oriented research
Physiology and metabolism
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Summary:Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. The total cytokinin activity was equivalent to 0.75 micrograms of kinetin per liter
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.55.1.266-267.1989