Peptides in common bean fractions inhibit human colorectal cancer cells

•Common bean peptides inhibited human colon cancer cell survival.•Two cultivars modified protein expression profile on human colon cancer cells.•Differences in protein expression correspond to cell cycle regulation and apoptosis. The aim of this study was to characterize peptides present in common b...

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Bibliographic Details
Published in:Food chemistry 2014-08, Vol.157, p.347-355
Main Authors: Luna Vital, Diego A., González de Mejía, Elvira, Dia, Vermont P., Loarca-Piña, Guadalupe
Format: Article
Language:English
Subjects:
Apoptosis
Bioactive peptides
Biological and medical sciences
Cell Cycle Checkpoints
Colorectal cancer
Colorectal Neoplasms - chemistry
Colorectal Neoplasms - metabolism
Common bean
Digestion
Fabaceae - chemistry
Food toxicology
HCT116 Cells
Humans
Medical sciences
Multiple tumors. Solid tumors. Tumors in childhood (general aspects)
Non-digestible fraction
Peptides
Toxicology
Tumors
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Summary:•Common bean peptides inhibited human colon cancer cell survival.•Two cultivars modified protein expression profile on human colon cancer cells.•Differences in protein expression correspond to cell cycle regulation and apoptosis. The aim of this study was to characterize peptides present in common bean non-digestible fractions (NDF) produced after enzymatic digestion and determine their antiproliferative action on human colorectal cancer cells. Five NDF peptides represented 70% of total protein (GLTSK, LSGNK, GEGSGA, MPACGSS and MTEEY) with antiproliferative activity on human colon cancer cells. Based on the antiproliferative effect, HCT116 cell line was most sensitive to bean Azufrado Higuera (IC50=0.53mg/ml) and RKO to Bayo Madero (IC50=0.51mg/ml) peptide extracts. Both cultivars increased significantly (p
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2014.02.050