Disorganized muscle protein-1 (DIM-1) of filarial parasite Brugia malayi : cDNA cloning, expression, purification, structural modeling and its potential as vaccine candidate for human filarial infection

Highlights • DIM-1bm gene of Brugia malayi was cloned and rDIM-1bm protein was expressed and purified. • DIM-1bm was similar to that of other nematodes and belongs to IgSF with 3 Ig domains. • Immunization with rDIM-1bm partially protected Mastomys coucha against infection. • rDIM-1bm immunization e...

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Bibliographic Details
Published in:Vaccine 2014-03, Vol.32 (15), p.1693-1699
Main Authors: Kushwaha, Vikas, Kumar, Vikash, Verma, Shiv K, Sharma, Rolee, Siddiqi, M.I, Murthy, P.K
Format: Article
Language:English
Subjects:
Allergy and Immunology
Amino Acid Sequence
Animals
Antigens, Helminth - chemistry
Antigens, Helminth - genetics
Antigens, Helminth - immunology
Applied microbiology
Biological and medical sciences
Brugia malayi
Brugia malayi - genetics
Cloning
Cloning, Molecular
Diseases caused by nematodes
Disorganized muscle protein-1
E coli
Experiments
Female
Filariases
Fundamental and applied biological sciences. Psychology
Gerbillinae
Helminthic diseases
IFN-γ
IgG and its subclasses
Immunization
Immunoglobulin G - blood
Infectious diseases
Interferon-gamma - immunology
Laboratory animals
Lymphatic filariases
Macrophage activity
Macrophages - immunology
Male
Medical sciences
Microbiology
Models, Molecular
Molecular Sequence Data
Murinae
Muscle Proteins - chemistry
Muscle Proteins - genetics
Muscle Proteins - immunology
Nematoda
Nitric oxide
Nitric Oxide - metabolism
Parasites
Parasitic diseases
Phagocytosis
Proteins
Scholarships & fellowships
Tropical diseases
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Description
Summary:Highlights • DIM-1bm gene of Brugia malayi was cloned and rDIM-1bm protein was expressed and purified. • DIM-1bm was similar to that of other nematodes and belongs to IgSF with 3 Ig domains. • Immunization with rDIM-1bm partially protected Mastomys coucha against infection. • rDIM-1bm immunization enhanced IFN-γ & NO release, macrophage activity & IgG1, and 2a & 2b. • DIM-1bm's lack of homology with human DIM-1 may be explored for its vaccine potential.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2014.01.064