Eco-friendly decoration of graphene oxide with biogenic silver nanoparticles: antibacterial and antibiofilm activity

• Published in: Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2014-02, Vol.16 (2), p.1-16, Article 2110
• Main Authors: de Faria, Andreia Fonseca, de Moraes, Ana Carolina Mazarin, Marcato, Priscyla Daniely, Martinez, Diego Stéfani Teodoro, Durán, Nelson, Filho, Antônio Gomes Souza, Brandelli, Adriano, Alves, Oswaldo Luiz
• Format: Article
• Language: English
• Subjects: Characterization and Evaluation of Materials; Chemistry and Materials Science; Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; Exact sciences and technology; Fullerenes and related materials; diamonds, graphite; Fusarium oxysporum; Inorganic Chemistry; Lasers; Materials Science; Methods of nanofabrication; Microorganisms; Nanocrystalline materials; Nanoparticles; Nanoscale materials and structures: fabrication and characterization; Nanotechnology; Optical Devices; Optics; Photonics; Physical Chemistry; Physics; Research Paper; Salmonella typhimurium; Silver; Solid surfaces and solid-solid interfaces; Specific materials; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties)
• ISSN: 1388-0764; 1572-896X
• DOI: 10.1007/s11051-013-2110-7

This work reports on preparation, characterization, and antibacterial activity of graphene oxide (GO) decorated with biogenic silver nanoparticles (Bio-AgNPs) produced by the fungus Fusarium oxysporum . This nanocomposite (Bio-GOAg) was prepared by an ex situ process through the physical mixture of a GO dispersion with the previously prepared Bio-AgNPs. The adsorption of the Bio-AgNPs onto the GO sheets was confirmed by transmission electron microscopy. The average size of the Bio-AgNPs anchored onto the GO surface was found to be 3.5 nm. The antibacterial activity of the Bio-GOAg nanocomposite against Gram-positive and Gram-negative microorganisms was investigated and a very promising result was found for the Gram-negative strains. In addition, the Bio-GOAg nanocomposite displayed a very strong biocidal activity against the Salmonella typhimurium strain at a concentration of 2.0 μg/mL. The antibiofilm activity toward S . typhimurium adhered on stainless steel surfaces was also investigated. The results showed 100 % inhibition of the adhered cells after exposure to the Bio-GOAg nanocomposite for 1 h.