CD200 has an important role in the differential diagnosis of mature B‐cell neoplasms by multiparameter flow cytometry

Background Multiparameter flow cytometry is a useful tool for the diagnostic evaluation of mature B‐cell neoplasms (MBN). Recently, it has been shown that CD200 may improve the distinction between chronic lymphocytic leukemia (CLL; CD200+) and mantle cell lymphoma (MCL; CD200−), but the role of CD20...

Full description

Saved in:
Bibliographic Details
Published in:Cytometry. Part B, Clinical cytometry Clinical cytometry, 2014-03, Vol.86 (2), p.98-105
Main Authors: Sandes, Alex F., de Lourdes Chauffaille, Maria, Oliveira, Cláudia Regina M.C., Maekawa, Yumi, Tamashiro, Nair, Takao, Tânia T., Ritter, Estela C., Rizzatti, Edgar G.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Antigens, CD - analysis
Antigens, CD - immunology
CD200
chronic lymphocytic leukemia
Diagnosis, Differential
Female
Flow Cytometry
Humans
Immunophenotyping
Leukemia, Lymphocytic, Chronic, B-Cell - diagnosis
Leukemia, Lymphocytic, Chronic, B-Cell - immunology
lymphoma
Lymphoma, Mantle-Cell - diagnosis
Lymphoma, Mantle-Cell - immunology
Male
mantle cell lymphoma
Middle Aged
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Multiparameter flow cytometry is a useful tool for the diagnostic evaluation of mature B‐cell neoplasms (MBN). Recently, it has been shown that CD200 may improve the distinction between chronic lymphocytic leukemia (CLL; CD200+) and mantle cell lymphoma (MCL; CD200−), but the role of CD200 expression in atypical CLL and other MBN remains to be established. Methods To address this issue, we investigated the expression of CD200 in 159 consecutive cases of MBN. Results CD200 was strongly expressed in CLL and was revealed to be an excellent marker to distinguish CLL from MCL, even in cases of atypical CLL. However, lack of CD200 was not an exclusive finding of MCL, being also observed in other MBNs. Furthermore, CD200 was highly expressed in hairy cell leukemia, being useful in the differential diagnosis of lymphomas with villous lymphocytes. Herein, we propose an algorithm to classify CD5+ MBNs based on the expression of CD200, CD11c, heavy chain immunoglobulins, and Matutes score. Conclusions These results expand the understanding of the CD200 expression in MBNs, giving further support for the inclusion of this marker in the routine investigation by flow cytometry. © 2013 International Clinical Cytometry Society
ISSN:1552-4949
1552-4957
DOI:10.1002/cyto.b.21128