Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals that are capable of inducing cell death. The present study evaluated the susceptibility of Streptococcus mutans and Lactobac...

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Bibliographic Details
Published in:Lasers in medical science 2014-03, Vol.29 (2), p.629-635
Main Authors: Araújo, N. C., Fontana, C. R., Bagnato, V. S., Gerbi, M. E. M.
Format: Article
Language:English
Subjects:
Anti-Infective Agents - therapeutic use
Antimicrobial agents
Bacteria
Biofilms
Biofilms - drug effects
Cell Survival - drug effects
Curcumin - pharmacology
Dental Caries - drug therapy
Dental Caries - microbiology
Dentistry
Equipment Design
Humans
Lactobacillus acidophilus
Lactobacillus acidophilus - drug effects
Lactobacillus acidophilus - physiology
Lasers
Medicine
Medicine & Public Health
Molar, Third - drug effects
Molar, Third - microbiology
Optical Devices
Optics
Original Article
Photochemotherapy - instrumentation
Photochemotherapy - methods
Photodynamic therapy
Photonics
Quantum Optics
Streptococcus mutans
Streptococcus mutans - drug effects
Streptococcus mutans - physiology
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Summary:Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals that are capable of inducing cell death. The present study evaluated the susceptibility of Streptococcus mutans and Lactobacillus acidophilus to PDT grown as multi-species in the biofilm phase versus in dentine carious lesions. A brain-heart infusion culture medium supplemented with 1 % glucose, 2 % sucrose, and 1 % young primary culture of L . acidophilus 10 8  CFU/mL and S . mutans 10 8  CFU/mL was used to develop multi-species biofilms and to induce caries on human dentine slabs. Five different concentrations of curcumin (0.75, 1.5, 3.0, 4.0, and 5.0 g/L) were used associated with 5.7 J/cm 2 light emission diode. Four different groups were analyzed L−D− (control group), L−D+ (drug group), L+D− (light group), and L+D+ (PDT group). ANOVA/Tukey’s tests were conducted to compare groups. A significant reduction ( p
ISSN:0268-8921
1435-604X
DOI:10.1007/s10103-013-1369-3