Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation

DNA methylation has the potential to be a clinically important biomarker in cancer. This communication reports a real-time and label-free biosensing strategy for DNA methylation detection in the cancer cell line. This has been achieved by using surface plasmon resonance biosensing combined with the...

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2014-04, Vol.50 (27), p.3585-3588
Main Authors: Carrascosa, Laura G, Sina, Abu Ali Ibn, Palanisamy, Ramkumar, Sepulveda, Borja, Otte, Marinus A, Rauf, Sakandar, Shiddiky, Muhamad J A, Trau, Matt
Format: Article
Language:English
Subjects:
Biosensing Techniques
DNA - chemistry
DNA Methylation
Humans
MCF-7 Cells
Molecular Probes
Sulfites - chemistry
Surface Plasmon Resonance
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DNA methylation has the potential to be a clinically important biomarker in cancer. This communication reports a real-time and label-free biosensing strategy for DNA methylation detection in the cancer cell line. This has been achieved by using surface plasmon resonance biosensing combined with the highly specific molecular inversion probe based amplification method, which requires only 50 ng of bisulfite treated genomic DNA.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc49607d