Characteristics of functioning of succinate dehydrogenase from flight muscles of the bumblebee Bombus terrestris (L.)

It was found that the succinate oxidation rate in mitochondria of flight muscles of Bombus terrestris L. increased by a factor of 2.15 after flying for 1 h. An electrophoretically homogenous preparation of succinate dehydrogenase with a specific activity of 7.14 U/mg protein and 81.55-fold purity wa...

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Bibliographic Details
Published in:Biology bulletin of the Russian Academy of Sciences 2013-09, Vol.40 (5), p.429-434
Main Authors: Gorbacheva, T. M, Syromyatnicov, M. Yu, Popov, V. N, Lopatin, A. V, Eprintsev, A. T, Fedorin, D. N
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Bombus terrestris
Cell Biology
Ecology
enzyme activity
flight
flight muscles
functional properties
hydrogen ions
insects
Life Sciences
mitochondria
molecular weight
muscle tissues
oxidation
salts
succinate dehydrogenase
succinic acid
Zoology
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Summary:It was found that the succinate oxidation rate in mitochondria of flight muscles of Bombus terrestris L. increased by a factor of 2.15 after flying for 1 h. An electrophoretically homogenous preparation of succinate dehydrogenase with a specific activity of 7.14 U/mg protein and 81.55-fold purity was isolated from B. terrestris flight muscles. It is shown that this enzyme is represented in the muscle tissue by only one isoform with R f = 0.24. The molecular weight of the native molecule and its subunits A and B was determined. The kinetic characteristics of succinate dehydrogenase (K ₘ = 0.33 mM) and the optimal concentration of hydrogen ions (pH 7.8) were established, and the effect of salts on the enzyme activity was studied. The role of succinate as a respiratory substrate in stress and the structural and functional characteristics of the succinate dehydrogenase system in the flight muscles of insects are discussed.
ISSN:1062-3590
1608-3059
DOI:10.1134/S1062359013050051