1-Deoxymannojirimycin inhibits Golgi-mediated processing of glycoprotein in Xenopus oocytes

The authors prepared in vitro an mRNA transcript coding for the erythroagglutinating subunit of the kidney bean glycoprotein phytohemagglutinin, E-PHA. The mRNA, injected into Xenopus oocytes, synthesized E-PHA carrying two Asn-linked carbohydrate chains, one of which was processed and acquired resi...

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Bibliographic Details
Published in:FEBS letters 1988-01, Vol.243 (2), p.489-492
Main Authors: Fabbrini, MS, Zoppe, M, Bollini, R, Vitale, A
Format: Article
Language:English
Subjects:
1-deoxymannojirimycin
Golgi apparatus
Xenopus
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Summary:The authors prepared in vitro an mRNA transcript coding for the erythroagglutinating subunit of the kidney bean glycoprotein phytohemagglutinin, E-PHA. The mRNA, injected into Xenopus oocytes, synthesized E-PHA carrying two Asn-linked carbohydrate chains, one of which was processed and acquired resistance to endo- beta -N-acetylglucosaminidase H, as occurs in the native bean cells. When the mannose analog 1-deoxymannojirimycin, an inhibitor of mammalian Golgi mannosidase I, was included in the oocyte culture medium, the acquisition of endo- beta -N-acetylglucosaminidase H resistance was abolished.
ISSN:0014-5793