Determination of vitamin C in drugs using of an optimized novel TCPOaAmplex redagold/silver alloy nanoparticlesaH2O2 chemiluminescence method by the BoxaBehnken design

Response surface methodology (RSM), based on a BoxaBehnken design (BBD) was used to optimize three of the most important operating variables (concentrations of TCPO, Amplex red and pH effect) at peroxyoxalate-chemiluminescence (PO-CL) system. For the first time Amplex red (AR) was introduced as a ne...

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Bibliographic Details
Published in:Journal of luminescence 2013-02, Vol.134, p.195-200
Main Authors: Chaichi, MJ, Alijanpour, SO
Format: Article
Language:English
Subjects:
Alloy systems
Design engineering
Drugs
Fluorescence
Nanostructure
Silver base alloys
Standard deviation
Vitamin C
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Summary:Response surface methodology (RSM), based on a BoxaBehnken design (BBD) was used to optimize three of the most important operating variables (concentrations of TCPO, Amplex red and pH effect) at peroxyoxalate-chemiluminescence (PO-CL) system. For the first time Amplex red (AR) was introduced as a new fluorescent emitter for predicting the reaction mechanism of PO-CL by means of the fluorescence property of its oxidation product. In optimum conditions, it was found that Au/Ag alloy nanoparticles (NPs) could enhance the CL intensity and the method sensitivity toward the evaluation of trace amount of vitamin C. Based on the antioxidant property of vitamin C noticeably the CL signal of the bis-(2,4,6-trichlorophenyl)oxalateaARaNPsahydrogen peroxideasodium salicylate system was quenched in a low basic medium. A simple, rapid and sensitive CL method for the determination of vitamin C has been developed. The results showed a linear relationship between vitamin C concentration and PO-CL intensity in the range of 0.082a82.7 mu g/mL. Detection limit of 0.012 mu g/mL and the relative standard deviation (RSD)
ISSN:0022-2313
DOI:10.1016/j.jlumin.2012.08.048