Highly selective fluorescence turn-on sensor for hydrogen sulfide and imaging in living cells

A displacement method of detecting hydrogen sulfide in aqueous media based on complex L–Cu ensemble is developed. Once combined with Cu2+, complex L–Cu displayed high specificity for sulfide anion. Among the various anions, only sulfide anion induce the revival of fluoresecence of compound L, which...

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Bibliographic Details
Published in:Journal of luminescence 2013-07, Vol.139, p.40-46
Main Authors: Wang, Jiaoliang, Long, Liping, Xie, Dan, Zhan, Youwen
Format: Article
Language:English
Subjects:
Anions
Cell imaging
Chemsensor
Condensed matter: electronic structure, electrical, magnetic, and optical properties
CuS
Dynamic response
Equivalence
Exact sciences and technology
Fluorescence
General equipment and techniques
Hydrogen sulfide
Imaging
Instruments, apparatus, components and techniques common to several branches of physics and astronomy
Luminescence
Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation
Photoluminescence
Physics
Sensors (chemical, optical, electrical, movement, gas, etc.)
remote sensing
Sulfides
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Summary:A displacement method of detecting hydrogen sulfide in aqueous media based on complex L–Cu ensemble is developed. Once combined with Cu2+, complex L–Cu displayed high specificity for sulfide anion. Among the various anions, only sulfide anion induce the revival of fluoresecence of compound L, which is quenched by Cu2+, resulting in turn-on type sensing sulfide anion. Complex L–Cu exhibits a highly sensitive fluorescent response toward S2− by releasing compound L to give a remarkable change with 20-fold fluorescence intensity enhancement under 2 equivalent of S2− added in Tris–HCl/ DMF (20mM, 6/4, v/v), and also exhibits a dynamic response range for S2− from 5×10−7 to 5×10−6M, with a detection limit of 0.18μM. In addition, the turn-on fluorescent change upon the addition of S2− is also applied in cell imaging. ► We have developed a displacement method with sensing hydrogen sulfide based on a complex L–Cu. ► It exhibits high selectivity, high sensitivity with a 20-fold fluorescence enhancement. ► It exhibited a low detection limit of 0.18μM. ► It is applied in vitro imaging of S2− in the living cells.
ISSN:0022-2313
1872-7883
DOI:10.1016/j.jlumin.2013.02.038