Isolation of genes encoding the Neurospora vacuolar ATPase. Analysis of vma-1 encoding the 67-kDa subunit reveals homology to other ATPases

The vacuolar membrane of Neurospora crassa contains a H+-translocating ATPase composed of at least three subunits with approximate molecular weights of 70,000, 60,000, and 15,000. Both genomic and cDNA clones encoding the largest subunit, which appears to contain the active site of the enzyme, have...

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Bibliographic Details
Published in:The Journal of biological chemistry 1988-10, Vol.263 (28), p.13994-14001
Main Authors: Bowman, E J, Tenney, K, Bowman, B J
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Amino Acid Sequence
Base Sequence
Biological and medical sciences
CHAMPIGNON
CODE GENETIQUE
CODIGO GENETICO
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
Fundamental and applied biological sciences. Psychology
FUNGI
Genes
Genes, Fungal
Genes. Genome
GENETIC CODE
HIDROLASAS
HYDROLASE
HYDROLASES
Macromolecular Substances
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Molecular Weight
Neurospora - genetics
NEUROSPORA CRASSA
Neurospora crassa - enzymology
Neurospora crassa - genetics
NUCLEOTIDE
NUCLEOTIDES
NUCLEOTIDOS
VACUOLA
VACUOLE
VACUOLES
Vacuoles - enzymology
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Summary:The vacuolar membrane of Neurospora crassa contains a H+-translocating ATPase composed of at least three subunits with approximate molecular weights of 70,000, 60,000, and 15,000. Both genomic and cDNA clones encoding the largest subunit, which appears to contain the active site of the enzyme, have been isolated and sequenced. The gene for this subunit, designated vma-1, contains six small introns (60-131 base pairs) and encodes a hydrophilic protein of 607 amino acids, Mr 67,121. Within the sequence is a putative nucleotide-binding region, consistent with the proposal that this subunit contains the site of ATP hydrolysis. This 67-kDa polypeptide shows high homology (62% identical residues overall and 84% in the middle of the protein) to the analogous polypeptide of a higher plant vacuolar ATPase. The hypothesis that the vacuolar ATPase is related to F0F1 ATPases is strongly supported by the finding of considerable homology between the 67-kDa subunit of the Neurospora vacuolar ATPase and both the alpha and beta subunits of F0F1 ATPases.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)68175-X