The preparation, characterization and evaluation of regenerated cellulose/collagen composite hydrogel films

• Published in: Carbohydrate polymers 2014-07, Vol.107, p.57-64
• Main Authors: Cheng, Yongmei, Lu, Jinting, Liu, Shilin, Zhao, Peng, Lu, Guozhong, Chen, Jinghua
• Format: Article
• Language: English
• Subjects: Animals; Applied sciences; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biological and medical sciences; Cellulose; Cellulose - analogs & derivatives; Cellulose - chemistry; Cellulose - pharmacology; Cellulose and derivatives; Collagen; Collagen - chemistry; Exact sciences and technology; Hydrogels - chemistry; Mechanical Phenomena; Medical sciences; Mice; Natural polymers; NIH 3T3 Cells; Physicochemistry of polymers; Porosity; Proteins; Scaffolds; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Tissue Engineering; Tissue Scaffolds - chemistry; Volatilization; Water - chemistry
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2014.02.034

•Production of DARC/Col film is relatively simple and cost efficient.•DARC/Col has 3D porous structure with excellent mechanical properties.•DARC/Col has good swelling behavior, moisture-penetrability and biocompatibility. Porous structured regenerated cellulose films were oxidized by periodate oxidation to obtain 2,3-dialdehyde cellulose (DARC) films, which were then reacted with collagen to obtain DARC/Col composite films. The subsequent FT-IR spectra indicated that collagen was immobilized on the DARC matrix via the Schiff base reaction between NH2 in collagen and CHO in DARC backbone. Scanning electron microscopy revealed that DARC/Col exhibited a refined 3D network structure and its porosity and pore size decreased with increasing of collagen concentration. The composite films demonstrated a good equilibrium-swelling ratio, air permeability and water retention properties. The composite films also showed excellent mechanical properties, which was vital for practical application. Finally, the cytotoxicity of the composite film was evaluated using NIH3T3 mice fibroblast cells, the results revealed that DARC/Col composite films have good biocompatibility for use as scaffold material in tissue engineering.