Structure of a homofructosan from Saussurea costus and anti-complementary activity of its sulfated derivatives

A homogeneous water-soluble polysaccharide APS-W1, (2→1)-β-d-fructofuranosan, with an average molecular weight of 3.9kDa, was isolated and characterized from the roots of Saussurea costus. Five sulfated derivatives of APS-W1 with different degrees of sulfation were prepared and they showed strong in...

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Bibliographic Details
Published in:Carbohydrate polymers 2014-05, Vol.105, p.152-160
Main Authors: HONGWEI FAN, FEI LIU, ANNIE BLIGH, S. W, SONGSHAN SHI, SHUNCHUN WANG
Format: Article
Language:English
Subjects:
Animals
Applied sciences
Biological and medical sciences
Blood Platelets - drug effects
Blood Platelets - metabolism
Complement Activation - drug effects
Complement Activation - physiology
Exact sciences and technology
Humans
Immunomodulators
Medical sciences
Natural polymers
Pharmacology. Drug treatments
Physicochemistry of polymers
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Plant Roots
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Rabbits
Saussurea
Sheep
Starch and polysaccharides
Sulfates - chemistry
Sulfates - isolation & purification
Sulfates - pharmacology
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Summary:A homogeneous water-soluble polysaccharide APS-W1, (2→1)-β-d-fructofuranosan, with an average molecular weight of 3.9kDa, was isolated and characterized from the roots of Saussurea costus. Five sulfated derivatives of APS-W1 with different degrees of sulfation were prepared and they showed strong inhibitory effect on the complement activation through the classical pathway (CP50: 2.2-18.9μg/mL; 8.3μg/mL for heparin) and alternative pathway (AP50: 11.4-115.8μg/mL; 89.2μg/mL for heparin). Mechanism studies by using complement-depleted sera indicated that sulfated derivatives with different positions of sulfation targeted to different complement proteins. Meanwhile the sulfated derivatives have limited anticoagulant effect based on re-calcification time and thrombin time. These results suggested that the sulfated derivatives prepared from APS-W1 could be promising potential complement inhibitors for the treatment of diseases caused by an over-activated complement system.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2014.01.084