Novel integrons and gene cassettes from a Cascadian submarine gas-hydrate-bearing core

Abstract To determine whether integrons are present in a submarine gas hydrate community, metagenomic DNA was extracted from a gas-hydrate-bearing core, 150 m below the seafloor, from the Cascadian Margin. Integrons and gene cassettes were recovered by PCR from metagenomic DNA and sequenced. Thirty-...

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Bibliographic Details
Published in:FEMS microbiology ecology 2014-02, Vol.87 (2), p.343-356
Main Authors: Elsaied, Hosam, Stokes, Hatch W., Yoshioka, Hideyoshi, Mitani, Yasuo, Maruyama, Akihiko
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Archaea
Archaea - genetics
Bacteria - genetics
Base Sequence
deep submarine gas hydrate
Deoxyribonucleic acid
Desulfuromonas acetoxidans
diversity
DNA
DNA, Archaeal - genetics
DNA, Bacterial - genetics
Ecology
gene cassettes
Genes, Archaeal
Genes, Bacterial
Geologic Sediments - microbiology
Integrases - genetics
integrons
Integrons - genetics
lateral cassette gene transfer
Marine
Methylomonas methanica
Microbial activity
Microbiology
Molecular Sequence Data
Ocean floor
Phylogeny
RNA, Ribosomal, 16S - genetics
structure
Sulfur
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Summary:Abstract To determine whether integrons are present in a submarine gas hydrate community, metagenomic DNA was extracted from a gas-hydrate-bearing core, 150 m below the seafloor, from the Cascadian Margin. Integrons and gene cassettes were recovered by PCR from metagenomic DNA and sequenced. Thirty-seven integron integrase phylotypes were identified. The phylotypes were diverse and included members with homology to integrases from Methylomonas methanica, Desulfuromonas acetoxidans, Thermodesulfatator indicus, and marine uncultured bacteria. The gene cassette composition, 153 gene cassettes, was dominated by two types of encoded putative proteins. The first of these was predicted oxidoreductases, such as iron/sulfur cluster-binding proteins. A second type was alkyl transferases. Some cassette proteins showed homologies with those from methane-related archaea. These observations suggest that integrons may assist in the adaptation of microbial communities in this environment.
ISSN:0168-6496
1574-6941
DOI:10.1111/1574-6941.12227