Virion RNA of beet yellows closterovirus: cell-free translation and some properties

1 Institute of Microbiology, 117811 Moscow and 2 Department of Virology and A. N. Belozersky Laboratory at Moscow State University, 119899 Moscow, U.S.S.R. Virion RNA of beet yellows virus (BYV) is a messenger-sense RNA of about 14500 nucleotides containing no poly(A) or covalently linked protein. I...

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Bibliographic Details
Published in:Journal of general virology 1989, Vol.70 (1), p.241-245
Main Authors: Karasev, A.V, Agranovsky, A.A, Rogov, V.V, Miroshnichenko, N.A, Dolja, V.V, Atabekov, J.G
Format: Article
Language:English
Subjects:
Beet yellows virus
Beta vulgaris
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
Myzus persicae
polypeptides
RNA
Tetragonia tetragonoides
Virology
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Summary:1 Institute of Microbiology, 117811 Moscow and 2 Department of Virology and A. N. Belozersky Laboratory at Moscow State University, 119899 Moscow, U.S.S.R. Virion RNA of beet yellows virus (BYV) is a messenger-sense RNA of about 14500 nucleotides containing no poly(A) or covalently linked protein. In vitro translation of BYV RNA yielded a prominent polypeptide of M r 250000, and some lighter products. None of the translation products was found to be immunoprecipitable with anti-BYV -globulins. The synthesis of all the BYV RNA-directed polypeptides was blocked by the cap analogue m 7 Gpp, thus suggesting the presence of a cap structure at the 5' end of virion RNA. Keywords: beet yellows virus, RNA, translation Received 13 July 1988; accepted 6 October 1988.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-70-1-241