Inhibitory effects of α-cyperone on adherence and invasion of avian pathogenic Escherichia coli O78 to chicken type II pneumocytes

• Published in: Veterinary immunology and immunopathology 2014-05, Vol.159 (1-2), p.50-57
• Main Authors: Zhang, Li-Yan, Lv, Shuang, Wu, Shuai-Cheng, Guo, Xun, Xia, Fang, Hu, Xi-Rou, Song, Zhou, Zhang, Cui, Qin, Qian-Qian, Fu, Ben-Dong, Yi, Peng-Fei, Shen, Hai-Qing, Wei, Xu-Bin
• Format: Article
• Language: English
• Subjects: Actin cytoskeleton polymerization; Adherence; Alveolar Epithelial Cells; Animals; APEC-O78; Bacterial Adhesion - drug effects; Cell Survival - drug effects; Chicken type II pneumocytes; Chickens; Escherichia coli - genetics; Escherichia coli - immunology; Escherichia coli - physiology; Escherichia coli Infections - immunology; Escherichia coli Infections - microbiology; Escherichia coli Infections - prevention & control; Escherichia coli Infections - veterinary; Humans; Invasion; Lung Diseases - immunology; Lung Diseases - microbiology; Lung Diseases - prevention & control; Lung Diseases - veterinary; Naphthalenes - pharmacology; Poultry Diseases - immunology; Poultry Diseases - microbiology; Poultry Diseases - prevention & control; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA, Bacterial - chemistry; RNA, Bacterial - genetics; Specific Pathogen-Free Organisms; α-cyperone
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2014.02.005

Avian pathogenic Escherichia coli (APEC) are extra-intestinal pathogenic E. coli, and usually cause avian septicemia through breaching the blood–gas barrier. Type II pneumocytes play an important role of maintaining the function of the blood–gas barrier. However, the mechanism of APEC injuring type II pneumocytes remains unclear. α-cyperone can inhibit lung cell injury induced by Staphylococcus aureus. In order to explore whether α-cyperone regulates the adherence and invasion of APEC-O78 to chicken type II pneumocytes, we successfully cultured chicken type II pneumocytes. The results showed that α-cyperone significantly decreased the adherence of APEC-O78 to chicken type II pneumocytes. In addition, α-cyperone inhibited actin cytoskeleton polymerization induced by APEC-O78 through down regulating the expression of Nck-2, Cdc42 and Rac1. These results provide new evidence for the prevention of colibacillosis in chicken.