Production of D-arabitol from raw glycerol by Candida quercitrusa

• Published in: Applied microbiology and biotechnology 2014-04, Vol.98 (7), p.2947-2953
• Main Authors: Yoshikawa, Jun, Habe, Hiroshi, Morita, Tomotake, Fukuoka, Tokuma, Imura, Tomohiro, Iwabuchi, Hiroyuki, Uemura, Shingo, Tamura, Takamitsu, Kitamoto, Dai
• Format: Article
• Language: English
• Subjects: Alcohol; Alcohols; Analysis; biodiesel; Biodiesel fuels; Biomedical and Life Sciences; Biosynthesis; Biotechnological Products and Process Engineering; Biotechnology; Biotransformation; calcium chloride; Candida; Candida - classification; Candida - genetics; Candida - isolation & purification; Candida - metabolism; Chromatography; DNA, Fungal - chemistry; DNA, Fungal - genetics; Environmental Microbiology; fermenters; Genetic aspects; Glycerol; Glycerol - metabolism; Life Sciences; Microbial Genetics and Genomics; Microbiological research; Microbiology; Microorganisms; Molecular Sequence Data; Phylogenetics; phylogeny; Properties; Raw materials; Sequence Analysis, DNA; Software; Studies; Sugar; Sugar Alcohols - metabolism; Yeast; yeast extract; Yeasts
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-013-5449-x

To promote the effective use of raw glycerol (a by-product of biodiesel production), 110 yeast strains that produce D-arabitol from glycerol were isolated from environmental samples. Among them, strain 17-2A was an effective D-arabitol producer in the presence of 250 g/l glycerol and was identified as Candida quercitrusa based on morphological, physicochemical, and phylogenetic analyses. C. quercitrusa type strain NBRC1022 produced the greatest quantity of D-arabitol (41.7 g/l) when the ability to produce D-arabitol from raw glycerol was compared among C. quercitrusa 17-2A and its phylogenetically related strains in flask culture. Under optimized culture conditions, strain NBRC1022 produced D-arabitol at a concentration of 58.2 g/l after a 7-day cultivation in 250 g/l glycerol, 6 g/l yeast extract, and 2 g/l CaCl₂. The culture conditions were further investigated with raw glycerol using a jar fermenter; the concentration of D-arabitol reached 67.1 g/l after 7 days and 85.1 g/l after 10 days, respectively, which corresponded to 0.40 g/g of glycerol. To our knowledge, the present D-arabitol yield from glycerol is higher than reported previously using microbial production.