Phenotypic and functional heterogeneity of CD169+ and CD163+ macrophages from porcine lymph nodes and spleen

•Macrophage subtypes in porcine secondary lympoid organs.•Receptors expressed on macrophages.•Functional analysis of macrophage subtypes. Secondary lymphoid organ macrophages are involved in the establishment of innate and acquired immunity. Here, we have isolated and characterized porcine lymph nod...

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Bibliographic Details
Published in:Developmental and comparative immunology 2014-05, Vol.44 (1), p.44-49
Main Authors: Alvarez, Belén, Martínez, Paloma, Yuste, María, Poderoso, Teresa, Alonso, Fernando, Domínguez, Javier, Ezquerra, Angel, Revilla, Concepción
Format: Article
Language:English
Subjects:
Animals
Antigen Presentation
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - metabolism
CD4-Positive T-Lymphocytes - immunology
Cells, Cultured
Lymph nodes
Lymph Nodes - cytology
Lymphocyte Activation
Macrophages
Macrophages - immunology
Phagocytosis
Phenotype
Pig
Receptors, Cell Surface - metabolism
Sialic Acid Binding Ig-like Lectin 1 - metabolism
Spleen
Spleen - cytology
Swine - immunology
Toll-Like Receptor 2 - metabolism
Toll-Like Receptor 4 - metabolism
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Summary:•Macrophage subtypes in porcine secondary lympoid organs.•Receptors expressed on macrophages.•Functional analysis of macrophage subtypes. Secondary lymphoid organ macrophages are involved in the establishment of innate and acquired immunity. Here, we have isolated and characterized porcine lymph node and spleen CD169+ and spleen CD163+ macrophages. Lymph node and spleen CD169+ macrophages can be both identified as CD172a+SLA-DRhiCD80/86hiCD14intTLR2+TLR4+. On the other side, spleen CD163+ macrophages are CD172a+SLA-DRintCD80/86intCD14−/loTLR2intTLR4int. In addition, these macrophages can be subdivided based on the expression of CD11R1 or CD11R3. Lymph node CD169+ macrophages phagocytozed polystyrene microspheres more efficiently than spleen CD163+ and CD169+ macrophages. All macrophages exhibited low capacity to take up and process the soluble antigen DQ-OVA. Finally, spleen CD163+ macrophages displayed the highest ability to present lysozyme to CD4+ T cells in a secondary in vitro response, followed by lymph node and spleen CD169+ macrophages.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2013.11.010