Modulation of lipocalin-type prostaglandin D2 synthase expression in catfish seminal vesicles by thyroid disrupting agents and hormones

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology 2013-11, Vol.158 (4), p.199-206
• Main Authors: Gunti, Sreenivasulu, Ayinampudi, Pavani, Chenichery, Sudhakumari, Aparna, Dutta-Gupta, Balasubramanian, Senthilkumaran
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; Base Sequence; biomarkers; Blotting, Northern; Catfish; Chorionic Gonadotropin - pharmacology; Clarias gariepinus; complementary DNA; Endocrine disruption; estradiol; Estradiol - pharmacology; Female; Fish Proteins - genetics; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic - drug effects; human chorionic gonadotropin; Humans; hyperthyroidism; Intramolecular Oxidoreductases - classification; Intramolecular Oxidoreductases - genetics; L-thyroxine; Lipocalins - classification; Lipocalins - genetics; Male; messenger RNA; Molecular Sequence Data; Northern blotting; overdose; Phylogeny; pollution; prostaglandin-D synthase; ptgds-b; reproduction; Reverse Transcriptase Polymerase Chain Reaction; Seminal vesicles; Seminal Vesicles - enzymology; Seminal Vesicles - growth & development; Seminal Vesicles - metabolism; sequence analysis; Sequence Homology, Amino Acid; testes; Testis - enzymology; Testis - growth & development; Testis - metabolism; Thiourea; Thiourea - pharmacology; Thyroid Gland - drug effects; Thyroid Gland - metabolism; Thyroxine; Thyroxine - pharmacology; Transcriptome - drug effects
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/j.cbpc.2013.08.002

Thyroid hormones play crucial role in several biological processes including reproduction. Disruption of normal thyroid status by environmental contaminants can cause severe impairment in reproductive functions. In our previous study, we reported down-regulation of a protein in seminal vesicular fluid of air-breathing catfish, Clarias gariepinus during experimentally induced hyperthyroidism. N-terminal amino acid sequence analysis followed by search in sequence database denoted it to be lipocalin-type prostaglandin D2 synthase (ptgds-b). In the present study, we cloned full-length cDNA of ptgds-b based on the N-terminal amino acid sequence. Surprisingly, Northern blot as well as RT-PCR analysis demonstrated the presence of ptgds-b transcript predominantly in seminal vesicles and developing testis. Further, ptgds-b mRNA significantly decreased in seminal vesicles following L-thyroxine overdose while there was an increased expression of ptgds-b after depletion of thyroid hormone by thiourea and withdrawal of the treatments reverted this effect. Treatment of catfish with human chorionic gonadotropin and estradiol significantly reduced ptgds-b expression. Taken together, we report ptgds-b as a thyroid hormone regulated protein in the seminal vesicles in addition to gonadotropin and estradiol. Further studies might explain the exclusive presence of ptgds-b in seminal vesicles and developing testis yet present data evaluated it as a putative biomarker for thyroid hormone disruption.