Oral immunization of fish against iridovirus infection using recombinant antigen produced from rice callus

Highlights • We expressed a recombinant major capsid protein (rMCP) of rock bream iridovirus in rice callus. • Fish immunized orally with rMCP underwent successful induction of antibodies and were protected against viral challenge. • Transgenic single-cell cultures offer advantages over whole-plant...

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Bibliographic Details
Published in:Vaccine 2013-10, Vol.31 (45), p.5210-5215
Main Authors: Shin, Y.J, Kwon, T.H, Seo, J.Y, Kim, T.J
Format: Article
Language:English
Subjects:
Administration, Oral
Algae
Allergy and Immunology
Animals
antibodies
Antibodies, Viral - blood
antigens
Antigens, Viral - genetics
Antigens, Viral - immunology
Antigens, Viral - isolation & purification
Biological and medical sciences
bream
callus
Cell culture
Chordata
Cloning
coat proteins
Deoxyribonucleic acid
DNA
DNA Virus Infections - prevention & control
DNA Virus Infections - veterinary
Endoplasmic reticulum
farmed fish
Fish diseases
Fish Diseases - prevention & control
freeze drying
Fundamental and applied biological sciences. Psychology
Gene amplification
genetically modified organisms
Immunization
Iridoviridae
Iridovirus
Iridovirus - genetics
Iridovirus - immunology
Methods
Microbiology
Molecular weight
morbidity
mortality
mucosal immunity
oral administration
Oral vaccine
Oryza - genetics
Oryza sativa
Plants, Genetically Modified
Proteins
Recombinant protein
rice
Rice callus
Rock bream iridovirus
Rocks
Studies
Vaccination - methods
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - genetics
Vaccines, Synthetic - immunology
Vaccines, Synthetic - isolation & purification
Viral diseases
Viral infections
Viral Vaccines - administration & dosage
Viral Vaccines - genetics
Viral Vaccines - immunology
Viral Vaccines - isolation & purification
Virology
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Summary:Highlights • We expressed a recombinant major capsid protein (rMCP) of rock bream iridovirus in rice callus. • Fish immunized orally with rMCP underwent successful induction of antibodies and were protected against viral challenge. • Transgenic single-cell cultures offer advantages over whole-plant systems with high level containment. • Plant-based oral administration protocol could be used as an effective (less labor and stress) means of accomplishing practical vaccine trials.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2013.08.085