G-CSF attenuates noise-induced hearing loss

• Published in: Neuroscience letters 2014-03, Vol.562, p.102-106
• Main Authors: Shi, Ze-tao, Lin, Ying, Wang, Jie, Wu, Jin, Wang, Ren-feng, Chen, Fu-quan, Mi, Wen-juan, Qiu, Jian-hua
• Format: Article
• Language: English
• Subjects: Animals; Cochlea; Cochlea - drug effects; Disease Models, Animal; Evoked Potentials, Auditory, Brain Stem - drug effects; Evoked Potentials, Auditory, Brain Stem - physiology; Female; G-CSF; Granulocyte Colony-Stimulating Factor - administration & dosage; Granulocyte Colony-Stimulating Factor - pharmacology; Guinea Pigs; Hair cell; Hair Cells, Auditory - drug effects; Hearing loss; Hearing Loss, Noise-Induced - drug therapy; Male; Noise; Noise induced hearing loss
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2013.07.033

•G-CSF can modestly alleviate experimental NIHL in guinea pigs.•G-CSF can protect hair cells in experimental NIHL model.•G-CSF can reduce the rate of missing hair cells in experimental NIHL model. In this study, we investigated the effects of granulocyte colony-stimulating factor (G-CSF) for the treatment of noise-induced hearing loss (NIHL) in a guinea pig model. Forty guinea pigs were randomly divided into four groups: control, noise (white noise, 3h/d for 2 days at 115dB), noise+G-CSF (350μg/kg/d for 5 days), and noise+saline. Auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) were used to determine the hearing threshold and outer hair cell function, respectively, in each group. Cochlear morphology was examined to evaluate hair cell injury induced by intense noise exposure. Fourteen days after noise exposure, the noise+G-CSF group had a lower ABR value than the noise group (P