Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering

Reconstructed liver has been desired as a liver substitute for transplantation. However, reconstruction of a whole liver has not been achieved because construction of a vascular network at an organ scale is very difficult. We focused on decellularized liver (DC-liver) as an artificial scaffold for t...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2013-12, Vol.116 (6), p.740-745
Main Authors: Shirakigawa, Nana, Takei, Takayuki, Ijima, Hiroyuki
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
blood
Collagen
Corrosion Casting
Decellularized liver
Endothelialization
Endothelium - cytology
engineering
Fundamental and applied biological sciences. Psychology
Hepatic Veins - physiology
Hepatocyte
hepatocytes
Hepatocytes - cytology
liver
Liver - blood supply
Liver - cytology
Liver tissue engineering
Liver, Artificial
Male
portal vein
Portal Vein - physiology
Rats
Rats, Wistar
Tissue Engineering - methods
Tissue Scaffolds
Vascular-tree network
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Summary:Reconstructed liver has been desired as a liver substitute for transplantation. However, reconstruction of a whole liver has not been achieved because construction of a vascular network at an organ scale is very difficult. We focused on decellularized liver (DC-liver) as an artificial scaffold for the construction of a hierarchical vascular network. In this study, we obtained DC-liver and the tubular network structure in which both portal vein and hepatic vein systems remained intact. Furthermore, endothelialization of the tubular structure in DC-liver was achieved, which prevented blood leakage from the tubular structure. In addition, hepatocytes suspended in a collagen sol were injected from the surroundings using a syringe as a suitable procedure for liver cell inoculation. In summary, we developed a base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2013.05.020