Photothermal therapy of tumors in lymph nodes using gold nanorods and near-infrared laser light

• Published in: Journal of controlled release 2013-12, Vol.172 (3), p.879-884
• Main Authors: Okuno, Tatsuki, Kato, Shigeki, Hatakeyama, Yuriko, Okajima, Junnosuke, Maruyama, Shigenao, Sakamoto, Maya, Mori, Shiro, Kodama, Tetsuya
• Format: Article
• Language: English
• Subjects: adverse effects; Animals; blood veins; Cell Line, Tumor; gold; Gold - chemistry; Gold - pharmacokinetics; Gold - therapeutic use; Gold nanorods; Humans; Hyperthermia, Induced; Infrared Rays; irradiation; Low-Level Light Therapy; Lymph node metastasis; lymph nodes; Lymph Nodes - drug effects; Lymph Nodes - pathology; Lymph Nodes - radiation effects; Lymphatic Metastasis - pathology; Lymphatic Metastasis - prevention & control; metastasis; Mice; monitoring; nanorods; Nanotubes - chemistry; neoplasm cells; neoplasms; Photothermal therapy; Plasmon resonance; tail; temperature; therapeutics
• ISSN: 0168-3659; 1873-4995
• DOI: 10.1016/j.jconrel.2013.10.014

Lymph node dissection for regional nodal metastasis is a primary option, but is invasive and associated with adverse effects. The development of non-invasive therapeutic methods in preclinical experiments using mice has been restricted by the small lymph node size and the limited techniques available for non-invasive monitoring of lymph node metastasis. Here, we show that photothermal therapy (PTT) using gold nanorods (GNRs) and near-infrared (NIR) laser light shows potential as a non-invasive treatment for tumors in the proper axillary lymph nodes (proper-ALNs) of MXH10/Mo–lpr/lpr mice, which develop systemic swelling of lymph nodes (up to 13mm in diameter, similar in size to human lymph nodes). Tumor cells were inoculated into the proper-ALNs to develop a model of metastatic lesions, and any anti-tumor effects of therapy were assessed. We found that GNRs accumulated in the tumor in the proper-ALNs 24h after tail vein injection, and that irradiation with NIR laser light elevated tumor temperature. Furthermore, combining local or systemic delivery of GNRs with NIR irradiation suppressed tumor growth more than irradiation alone. We propose that PTT with GNRs and NIR laser light can serve as a new therapeutic method for lymph node metastasis, as an alternative to lymph node dissection. [Display omitted]