New model for adenine-induced chronic renal failure in mice, and the effect of gum acacia treatment thereon: Comparison with rats

• Published in: Journal of pharmacological and toxicological methods 2013-11, Vol.68 (3), p.384-393
• Main Authors: Ali, Badreldin H., Al-Salam, Suhail, Al Za'abi, Mohammed, Waly, Mostafa I., Ramkumar, Aishwarya, Beegam, Sumyia, Al-Lawati, Intisar, Adham, Sirin A., Nemmar, Abderrahim
• Format: Article
• Language: English
• Subjects: Acacia; Adenine; Adenine - administration & dosage; Adenine - toxicity; Animal model; Animals; Chronic renal failure; Creatinine - blood; Disease Models, Animal; Dose-Response Relationship, Drug; Glutathione - metabolism; Gum Arabic - pharmacology; Kidney Failure, Chronic - drug therapy; Kidney Failure, Chronic - physiopathology; Male; Mice; Mice, Inbred C57BL; Rats; Rats, Wistar; Severity of Illness Index; Species Specificity; Superoxide Dismutase - metabolism; Urea - metabolism
• ISSN: 1056-8719; 1873-488X
• DOI: 10.1016/j.vascn.2013.05.001

This study aimed at comparing the effects of feeding mice and rats with adenine to induce a state of chronic renal failure (CRF), and to assess the effect of treatment with gum acacia (GA) thereon. We compared the outcome, in mice, of feeding adenine at three different doses (0.75%, 0.3%, and 0.2%, w/w). Biochemical and histopathological studies were conducted in plasma, urine and renal homogenates from both species. When mice and rats were fed adenine (0.75%, w/w), all treated rats survived the treatment, but all treated mice died within 1–2days. The dosage in mice was reduced to 0.3%, w/w, for 4weeks, but again all treated mice died within 3–4days. A further reduction in the dosage in mice to 0.2%, w/w, for 4weeks resulted in no mortality, and produced alterations similar to those observed in rats fed adenine at a dose of 0.75%,w/w, for 4weeks. Plasma creatinine, urea and urinary protein were significantly increased (P